PMID- 27619842
OWN - NLM
STAT- MEDLINE
DCOM- 20171222
LR  - 20180209
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 225
DP  - 2016 Oct 2
TI  - Genomic characterization and pathogenicity of a strain of type 1 porcine 
      reproductive and respiratory syndrome virus.
PG  - 40-49
LID - S0168-1702(16)30103-4 [pii]
LID - 10.1016/j.virusres.2016.09.006 [doi]
AB  - The emergence of type 1 porcine reproductive and respiratory syndrome virus 
      (PRRSV) has been noticed recently in China. In the present study, the complete 
      genomic characterization of a strain of type 1 PRRSV (designated GZ11-G1) was 
      described and its pathogenicity for piglets was analyzed. The results showed that 
      the complete genome of GZ11-G1 with a size of 15,094 nt, excluding the poly (A) 
      tails, shared 80.2-96.3% identity with the representative strains of type 1 
      PRRSV, and in particular, it had highest homology (96.3%) with Amervac PRRS, a 
      live vaccine virus of type 1 PRRSV and SHE, a rescued virus from an infectious 
      clone of Amervac PRRS virus. Compared with the vaccine virus, the nonstructural 
      and structural proteins of GZ11-G1 displayed extensive amino acid variations 
      except for its ORF5a. GZ11-G1 was clustered with the strains of type 1 PRRSV 
      including Cresa3267, Cresa3249, Cresa3256, Olot/91, 9625/2012, ESP-1991-Olot91 
      and Amervac PRRS vaccine virus by further phylogenetic analysis. Moreover, 
      GZ11-G1 was shown to cause fever, higher viremia and lung and lymph node lesions 
      in piglets. Our findings indicate that GZ11-G1 is genetically related to type 1 
      PRRSV strains within the cluster formed by Cresa3267, Cresa3249, Cresa3256, 
      Olot/91, 9625/2012, ESP-1991-Olot91 and Amervac PRRS vaccine virus, and it is a 
      pathogenic for piglets. This study aids in understanding the genetic variation 
      and evolution of type 1 PRRSV.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Wang, Xingchen
AU  - Wang X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China.
FAU - Yang, Xiaorong
AU  - Yang X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China.
FAU - Zhou, Rong
AU  - Zhou R
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China. Electronic 
      address: guoxin@cau.edu.cn.
FAU - Yang, Hanchun
AU  - Yang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing 100193, People's Republic of China. Electronic 
      address: yanghanchun1@cau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160913
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Genetic Variation
MH  - *Genome, Viral
MH  - *Genomics
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/*genetics/pathogenicity
MH  - RNA, Viral
MH  - Swine
MH  - Viral Load
OTO - NOTNLM
OT  - Genome
OT  - Pathogenicity
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Type 1
EDAT- 2016/09/14 06:00
MHDA- 2017/12/23 06:00
CRDT- 2016/09/14 06:00
PHST- 2016/02/08 00:00 [received]
PHST- 2016/08/24 00:00 [revised]
PHST- 2016/09/08 00:00 [accepted]
PHST- 2016/09/14 06:00 [pubmed]
PHST- 2017/12/23 06:00 [medline]
PHST- 2016/09/14 06:00 [entrez]
AID - S0168-1702(16)30103-4 [pii]
AID - 10.1016/j.virusres.2016.09.006 [doi]
PST - ppublish
SO  - Virus Res. 2016 Oct 2;225:40-49. doi: 10.1016/j.virusres.2016.09.006. Epub 2016 
      Sep 13.