PMID- 27620818
OWN - NLM
STAT- MEDLINE
DCOM- 20170322
LR  - 20170923
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 58
DP  - 2016 Nov
TI  - Identification and functional characterization of tumor necrosis factor receptor 
      1 (TNFR1) of grass carp (Ctenopharyngodon idella).
PG  - 24-32
LID - S1050-4648(16)30551-4 [pii]
LID - 10.1016/j.fsi.2016.09.003 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) exerts its regulatory effects by binding one 
      of two TNF receptors, TNF-alpha receptor 1 (TNFR1) or TNFR2. In this study, we 
      isolated and identified the cDNA sequence of grass carp TNFR1 (gcTNFR1). Similar 
      to its homologs in other fish species, the putative protein of gcTNFR1 possessed 
      an extracellular region containing three TNF homology domains, a transmembrane 
      region and a cytoplasmic region with a conserved death domain. Consistent with 
      the widespread expression of mammalian TNFR1, gcTNFR1 transcripts ubiquitously 
      expressed in spleen, thymus, liver, heart, gill, intestine, brain and head kidney 
      with the highest expression levels in head kidney. To reveal its inductive 
      expression patterns in inflammatory response, effect of in vivo bacterial 
      infection on gcTNFR1 gene expression was examined, showing a rapid increase of 
      gcTNFR1 expression in head kidney, gill, liver and intestine, which is consistent 
      with the role of TNF-alpha as an early response gene during immune challenges. To 
      define the functional role of gcTNFR1, recombinant extracellular region of 
      gcTNFR1 (rgcTNFR1) was prepared and used to perform in vitro binding assay, 
      demonstrating its ability to interact with recombinant grass carp TNF-alpha 
      (rgcTNF-alpha). Furthermore, to characterize the function of gcTNFR1 in affecting 
      rgcTNF-alpha actions, the effect of overexpressing gcTNFR1 on rgcTNF-alpha-induced grass 
      carp IL-1beta (gcIL-1beta) promoter activity was determined in COS7 cells. Results 
      showed that gcTNFR1 was involved in the regulation of rgcTNF-alpha on gcIL-1beta 
      transcription. Consistently, rgcTNFR1 was effective in attenuating the effect of 
      rgcTNF-alpha on IL-1beta mRNA expression in grass carp head kidney leukocytes, providing 
      evidence for the involvement of TNFR1 in TNF-alpha signaling in grass carp. These 
      data facilitate a better understanding of TNF-alpha receptor signaling in grass carp.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Zhang, Shengnan
AU  - Zhang S
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Ma, Tengyue
AU  - Ma T
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Qiu, Xingyang
AU  - Qiu X
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Zhang, Anying
AU  - Zhang A
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, Center for Informational Biology, 
      University of Electronic Science and Technology of China, Chengdu 610054, 
      People's Republic of China. Electronic address: zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160909
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
SB  - IM
MH  - Aeromonas hydrophila/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Carps/classification/genetics/immunology
MH  - DNA, Complementary/genetics/metabolism
MH  - Fish Diseases/*genetics/immunology/microbiology
MH  - Fish Proteins/chemistry/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Gram-Negative Bacterial Infections/genetics/immunology/microbiology/*veterinary
MH  - Head Kidney/immunology
MH  - Immunity, Innate/genetics
MH  - Leukocytes/immunology
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I/chemistry/*genetics/metabolism
MH  - Sequence Alignment
OTO - NOTNLM
OT  - Binding assay
OT  - Functional identification
OT  - Grass carp
OT  - Molecular characterization
OT  - TNF-alpha receptor 1
EDAT- 2016/10/23 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/09/14 06:00
PHST- 2016/06/24 00:00 [received]
PHST- 2016/08/14 00:00 [revised]
PHST- 2016/09/08 00:00 [accepted]
PHST- 2016/10/23 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
PHST- 2016/09/14 06:00 [entrez]
AID - S1050-4648(16)30551-4 [pii]
AID - 10.1016/j.fsi.2016.09.003 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Nov;58:24-32. doi: 10.1016/j.fsi.2016.09.003. Epub 
      2016 Sep 9.