PMID- 27621952
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160913
LR  - 20220321
IS  - 2157-6998 (Print)
IS  - 2157-7005 (Electronic)
IS  - 2157-7005 (Linking)
VI  - 6
IP  - 3
DP  - 2016 Jul
TI  - Neurological Outcomes after Human Umbilical Cord Patch for In Utero Spina Bifida 
      Repair in a Sheep Model.
PG  - e309-17
LID - 10.1055/s-0036-1592316 [doi]
AB  - OBJECTIVES: The objective of our study was to test the hypothesis that in utero 
      repair of surgically created spina bifida in a sheep model using cryopreserved 
      human umbilical cord (HUC) patch improves neurological outcome. METHODS: Spina 
      bifida with myelotomy was surgically created in timed pregnant ewes at 
      gestational day (GD) 75. The fetuses were randomly assigned to unrepaired versus 
      HUC and treated at GD 95 and then delivered at GD 140. Neurological evaluation 
      was performed using the Texas Spinal Cord Injury Scale (TSCIS), bladder control 
      using ultrasound, and the hindbrain herniation. RESULTS: Three lambs without the 
      spina bifida creation served as controls. There were four lambs with spina 
      bifida: two were unrepaired and two underwent HUC repair. The control lambs had 
      normal function. Both unrepaired lambs had nonhealed skin lesions with leakage of 
      cerebrospinal fluid, a 0/20 TSCIS score, no bladder control, and the hindbrain 
      herniation. In contrast, both HUC lambs had a completely healed skin defect and 
      survived to day 2 of life, a 3/20 and 4/20 TSCIS score (nociception), partial 
      bladder control, and normal hindbrain anatomy. CONCLUSIONS: Cryopreserved HUC 
      patch appears to improve survival and neurological outcome in this severe form of 
      the ovine model of spina bifida.
FAU - Papanna, Ramesha
AU  - Papanna R
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and 
      Reproductive Medicine, UTHealth, The University of Texas Health Science at 
      Houston, McGovern Medical School and the Fetal Center at Children's Memorial 
      Hermann Hospital, Houston, Texas.
FAU - Mann, Lovepreet K
AU  - Mann LK
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and 
      Reproductive Medicine, UTHealth, The University of Texas Health Science at 
      Houston, McGovern Medical School and the Fetal Center at Children's Memorial 
      Hermann Hospital, Houston, Texas.
FAU - Snowise, Saul
AU  - Snowise S
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and 
      Reproductive Medicine, UTHealth, The University of Texas Health Science at 
      Houston, McGovern Medical School and the Fetal Center at Children's Memorial 
      Hermann Hospital, Houston, Texas.
FAU - Morales, Yisel
AU  - Morales Y
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and 
      Reproductive Medicine, UTHealth, The University of Texas Health Science at 
      Houston, McGovern Medical School and the Fetal Center at Children's Memorial 
      Hermann Hospital, Houston, Texas.
FAU - Prabhu, Sanjay P
AU  - Prabhu SP
AD  - Department of Radiology, Harvard Medical School, Boston Children's Hospital, 
      Boston, Massachusetts.
FAU - Tseng, Scheffer C G
AU  - Tseng SC
AD  - The Ocular Surface Center, Miami, Florida; TissueTech, Inc., Miami, Florida.
FAU - Grill, Raymond
AU  - Grill R
AD  - Department of Neurobiology and Anatomical Sciences, University of Mississippi 
      Medical Center, Jackson, Mississippi.
FAU - Fletcher, Stephen
AU  - Fletcher S
AD  - Division of Pediatric Neurosurgery, The Department of Pediatrics, UTHealth, The 
      University of Texas Health Science at Houston, McGovern Medical School, Houston, 
      Texas; Department of Pediatric Surgery, UTHealth, The University of Texas Health 
      Science at Houston, McGovern Medical School, Houston, Texas.
FAU - Moise, Kenneth J Jr
AU  - Moise KJ Jr
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology and 
      Reproductive Medicine, UTHealth, The University of Texas Health Science at 
      Houston, McGovern Medical School and the Fetal Center at Children's Memorial 
      Hermann Hospital, Houston, Texas.
LA  - eng
PT  - Case Reports
PL  - United States
TA  - AJP Rep
JT  - AJP reports
JID - 101569862
PMC - PMC5017885
OTO - NOTNLM
OT  - neural tube defect
OT  - regenerative healing
OT  - sheep spina bifida model
OT  - spina bifida repair
OT  - umbilical cord
EDAT- 2016/09/14 06:00
MHDA- 2016/09/14 06:01
PMCR- 2016/07/01
CRDT- 2016/09/14 06:00
PHST- 2016/09/14 06:00 [entrez]
PHST- 2016/09/14 06:00 [pubmed]
PHST- 2016/09/14 06:01 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 160051 [pii]
AID - 10.1055/s-0036-1592316 [doi]
PST - ppublish
SO  - AJP Rep. 2016 Jul;6(3):e309-17. doi: 10.1055/s-0036-1592316.