PMID- 27623942
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 11
DP  - 2016 Nov
TI  - Global methylation profiling to identify epigenetic signature of gallbladder 
      cancer and gallstone disease.
PG  - 14687-14699
AB  - Promoter methylation in various tumor suppressor genes is reported to influence 
      gallbladder carcinogenesis. Here, we aimed to identify methylation status in 
      gallbladder cancer (GBC) by performing a comprehensive genome-wide DNA 
      methylation profiling. The methylation status of 485,577 CpG sites were 
      investigated using Illumina's Infinium Human Methylation 450 BeadChip array in 24 
      tissues (eight each of tumor, adjacent non-tumor, and gallstone). About 33,443 
      differentially methylated sites (DMRs) were obtained in the whole human genome, 
      of which 24,188 (72 %) were hypermethylated and 9255 (28 %) were hypomethylated. 
      The data also revealed that majority of the DMRs are localized on the proximal 
      promoter region [Transcription start sites (TSS200, TSS1500) and 5' untranslated 
      region (5'UTR)] and first exon. Exclusion of first exon detected a total of 
      10,123 (79 %) hypermethylated and 2703 (21 %) hypomethylated sites. Comparative 
      analysis of the later with our differential proteomics data resulted 
      in identification of 7 hypermethylated or down-regulated (e.g., FBN1, LPP, and 
      SOD3) and 61 hypomethylated or up-regulated markers (e.g., HBE1, SNRPF, TPD52) 
      for GBC. These genes could be further validated on the basis of their 
      methylation/expression status in order to identify their utility to be used as 
      biomarker/s for early diagnosis and management of GBC.
FAU - Sharma, Preeti
AU  - Sharma P
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India.
FAU - Bhunia, Shushruta
AU  - Bhunia S
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India.
FAU - Poojary, Satish S
AU  - Poojary SS
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India.
FAU - Tekcham, Dinesh S
AU  - Tekcham DS
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India.
FAU - Barbhuiya, Mustafa Ahmed
AU  - Barbhuiya MA
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India.
AD  - The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of 
      Medicine, MD, Baltimore, USA.
FAU - Gupta, Sanjiv
AU  - Gupta S
AD  - Cancer Hospital and Research Institute, Cancer Hills, Gwalior, Madhya Pradesh, 
      474009, India.
FAU - Shrivastav, Braj Raj
AU  - Shrivastav BR
AD  - Cancer Hospital and Research Institute, Cancer Hills, Gwalior, Madhya Pradesh, 
      474009, India.
FAU - Tiwari, Pramod Kumar
AU  - Tiwari PK
AD  - Molecular and Human Genetics Division, Centre for Genomics, Jiwaji University, 
      Gwalior, Madhya Pradesh, 474011, India. pk_tiwari@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160914
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - CpG Islands/genetics
MH  - *DNA Methylation
MH  - Epigenomics
MH  - Female
MH  - Follow-Up Studies
MH  - Gallbladder Neoplasms/*genetics/pathology
MH  - Gallstones/*genetics/pathology
MH  - *Gene Expression Profiling
MH  - *Genome, Human
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Prognosis
MH  - Promoter Regions, Genetic/*genetics
MH  - Transcription Initiation Site
OTO - NOTNLM
OT  - DNA methylation
OT  - Gallbladder cancer
OT  - Gallstone disease
OT  - Infinium 450K BeadChip
OT  - Methylome profile
EDAT- 2016/09/15 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/09/15 06:00
PHST- 2016/04/02 00:00 [received]
PHST- 2016/09/07 00:00 [accepted]
PHST- 2016/09/15 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
PHST- 2016/09/15 06:00 [entrez]
AID - 10.1007/s13277-016-5355-9 [pii]
AID - 10.1007/s13277-016-5355-9 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Nov;37(11):14687-14699. doi: 10.1007/s13277-016-5355-9. Epub 
      2016 Sep 14.