PMID- 27623952
OWN - NLM
STAT- MEDLINE
DCOM- 20170201
LR  - 20181113
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 12
IP  - 1
DP  - 2016 Sep 13
TI  - Changes in biochemical analytes in female dogs with subclinical Ancylostoma spp. 
      infection.
PG  - 203
LID - 10.1186/s12917-016-0833-2 [doi]
LID - 203
AB  - BACKGROUND: Ancylostoma spp. is one of the most prevalent canine intestinal 
      nematode infections which usually causes subclinical disease in adult dogs and 
      has zoonotic implications. Therefore, the aim of this study was to explore and 
      evaluate the possible pathophysiological changes that Ancylostoma spp. could 
      produce in female dogs naturally infected but without clinical signs of disease, 
      by screening a wide variety of biochemical markers for potential changes. Samples 
      of feces and blood of 45 dogs were collected and fecal flotation and zinc 
      sulphate centrifugal flotation were performed. The biochemical analytes 
      determined were: the acute-phase proteins C-reactive protein (CRP) and 
      haptoglobin (Hp); the lipid profile (cholesterol, triglycerides, HDL, LDL); the 
      serum iron profile: iron, unsaturated iron binding-capacity (UIBC), and ferritin; 
      the enzyme butyrylcholinesterase (BChe); the pancreatic profile: amylase, lipase, 
      and trypsin-like immunoreactivity (TLI); the oxidative stress markers: total 
      antioxidant capacity (TAC) and paraoxonase -1 (PON-1), along with total protein, 
      albumin, and insulin-like growth factor - 1 (IGF - 1). Ancylostoma spp. eggs were 
      detected in 29/45 dogs (64.4 %). Dogs were divided into two groups according to 
      the results of fecal flotation methods. Group 1: negative fecal floatation 
      (n = 16), and Group 2: subclinical infection with the observation of Ancylostoma 
      spp. type eggs/x 40 objective fields (n = 29). RESULTS: Mann-Whitney U test was 
      used to compare the biochemical analyte results between the two groups 
      (P < 0.05). Significant increases in CRP (mug/mL) (median): non-infected dogs: 
      5.5; subclinically infected dogs 18.7; P = 0.03, Hp (g/L) (median): G1: 2.4; G2: 
      3.3; P = 0.03, and UIBC (mug/dL) (median): non-infected dogs: 139.4; subclinically 
      infected dogs: 216; P = 0.0015, and significantly decreased iron (mug/dL) 
      (median): non-infected dogs: 202.5; subclinically infected dogs: 125.7; 
      P = 0.0041, IGF-1 (ng/mL) (median): non-infected dogs: 224; subclinically 
      infected dogs: 123; P = 0.02, and albumin (g/dL) (median): non-infected dogs: 
      2.8; subclinically infected dogs: 2.5; P = 0.04 concentrations were observed in 
      dogs with subclinical Ancylostoma spp. infection when compared to non-infected 
      dogs. CONCLUSION: These findings provide an overview of the biochemical effects 
      produced by patent Ancylostoma spp. in naturally infected dogs without any 
      evident clinical signs of disease, which could be considered in differential 
      diagnosis, especially in an endemic area for this parasite.
FAU - Schmidt, Elizabeth M S
AU  - Schmidt EM
AD  - Department of Veterinary Clinical Sciences, School of Veterinary Medicine and 
      Animal Science, Sao Paulo State University (FMVZ -UNESP), Campus Botucatu. 
      Distrito de Rubiao Jr, s/n. 18.618-000, Botucatu, SP, Brazil.
FAU - Tvarijonaviciute, Asta
AU  - Tvarijonaviciute A
AD  - Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, Campus of 
      Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
FAU - Martinez-Subiela, Silvia
AU  - Martinez-Subiela S
AD  - Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, Campus of 
      Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
FAU - Ceron, Jose J
AU  - Ceron JJ
AD  - Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, Campus of 
      Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
FAU - Eckersall, Peter D
AU  - Eckersall PD
AUID- ORCID: 0000-0001-5254-5502
AD  - Institute of Biodiversity, Animal Health and Comparative Medicine, College of 
      Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. 
      David.Eckersall@glasgow.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20160913
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
SB  - IM
MH  - *Ancylostoma
MH  - Ancylostomiasis/blood/*veterinary
MH  - Animals
MH  - Dog Diseases/blood/*parasitology
MH  - Dogs
MH  - Feces/parasitology
MH  - Female
MH  - Ovum
PMC - PMC5022191
OTO - NOTNLM
OT  - Acute phase proteins
OT  - C-reactive protein
OT  - Haptoglobin
OT  - Hookworm
OT  - IGF-1
OT  - Iron
EDAT- 2016/09/15 06:00
MHDA- 2017/02/02 06:00
PMCR- 2016/09/13
CRDT- 2016/09/15 06:00
PHST- 2015/07/30 00:00 [received]
PHST- 2016/09/06 00:00 [accepted]
PHST- 2016/09/15 06:00 [entrez]
PHST- 2016/09/15 06:00 [pubmed]
PHST- 2017/02/02 06:00 [medline]
PHST- 2016/09/13 00:00 [pmc-release]
AID - 10.1186/s12917-016-0833-2 [pii]
AID - 833 [pii]
AID - 10.1186/s12917-016-0833-2 [doi]
PST - epublish
SO  - BMC Vet Res. 2016 Sep 13;12(1):203. doi: 10.1186/s12917-016-0833-2.