PMID- 27626665
OWN - NLM
STAT- MEDLINE
DCOM- 20171113
LR  - 20181113
IS  - 2211-1247 (Electronic)
VI  - 16
IP  - 11
DP  - 2016 Sep 13
TI  - Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface 
      Markers and Differential Inflammasome Function.
PG  - 2953-2966
LID - S2211-1247(16)31070-1 [pii]
LID - 10.1016/j.celrep.2016.08.023 [doi]
AB  - Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. 
      In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ 
      and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. 
      Although RNA-expression profiles have been previously compared, protein 
      expression data may provide a different picture. Here, we exploited label-free 
      quantitative mass spectrometry to compare and identify differences in primary 
      human DC subset proteins. Moreover, we integrated these proteomic data with 
      existing mRNA data to derive robust cell-specific expression signatures with more 
      than 400 differentially expressed proteins between subsets, forming a solid basis 
      for investigation of subset-specific functions. We illustrated this by extracting 
      subset identification markers and by demonstrating that pDCs lack caspase-1 and 
      only express low levels of other inflammasome-related proteins. In accordance, 
      pDCs were incapable of interleukin (IL)-1beta secretion in response to ATP.
CI  - Copyright (c) 2016 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Worah, Kuntal
AU  - Worah K
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Mathan, Till S M
AU  - Mathan TSM
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Vu Manh, Thien Phong
AU  - Vu Manh TP
AD  - Centre d'Immunologie, de Marseille-Luminy, Aix Marseille University UM2, Inserm, 
      U1104, CNRS UMR7280, Cedex 09, 13288 Marseille, France.
FAU - Keerthikumar, Shivakumar
AU  - Keerthikumar S
AD  - Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University 
      Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the Netherlands.
FAU - Schreibelt, Gerty
AU  - Schreibelt G
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Tel, Jurjen
AU  - Tel J
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Duiveman-de Boer, Tjitske
AU  - Duiveman-de Boer T
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Skold, Annette E
AU  - Skold AE
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands; Department of Oncology-Pathology, Karolinska University Hospital 
      Solna, Karolinska Institutet, 171 76 Stockholm, Sweden.
FAU - van Spriel, Annemiek B
AU  - van Spriel AB
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - de Vries, I Jolanda M
AU  - de Vries IJM
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands; Department of Medical Oncology, Radboud Institute for Molecular Life 
      Sciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands.
FAU - Huynen, Martijn A
AU  - Huynen MA
AD  - Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University 
      Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the Netherlands.
FAU - Wessels, Hans J
AU  - Wessels HJ
AD  - Radboud Proteomics Centre, Department of Laboratory Medicine, Radboud University 
      Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the Netherlands; Nijmegen Centre 
      for Mitochondrial Disorders, Radboud University Medical Center, P.O. Box 9101, 
      6500 HB Nijmegen, the Netherlands.
FAU - Gloerich, Jolein
AU  - Gloerich J
AD  - Radboud Proteomics Centre, Department of Laboratory Medicine, Radboud University 
      Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the Netherlands.
FAU - Dalod, Marc
AU  - Dalod M
AD  - Centre d'Immunologie, de Marseille-Luminy, Aix Marseille University UM2, Inserm, 
      U1104, CNRS UMR7280, Cedex 09, 13288 Marseille, France.
FAU - Lasonder, Edwin
AU  - Lasonder E
AD  - Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University 
      Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the Netherlands; School of 
      Biomedical and Healthcare Sciences, B427 Portland Square, Plymouth University, 
      Drake Circus, Plymouth, Devon PL4 8AA, UK.
FAU - Figdor, Carl G
AU  - Figdor CG
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands. Electronic address: carl.figdor@radboudumc.nl.
FAU - Buschow, Sonja I
AU  - Buschow SI
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, the 
      Netherlands; Department of Gastroenterology and Hepatology, Erasmus MC-University 
      Medical Center, 3015 CN Rotterdam, the Netherlands. Electronic address: 
      s.buschow@erasmusmc.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Biomarkers)
RN  - 0 (Inflammasomes)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Biomarkers/*metabolism
MH  - Caspase 1/metabolism
MH  - Dendritic Cells/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inflammasomes/*metabolism
MH  - Protein Interaction Maps
MH  - Proteomics/*methods
MH  - Reproducibility of Results
MH  - Transcriptome/genetics
PMC - PMC5039226
EDAT- 2016/09/15 06:00
MHDA- 2017/11/14 06:00
PMCR- 2016/09/13
CRDT- 2016/09/15 06:00
PHST- 2015/12/21 00:00 [received]
PHST- 2016/06/25 00:00 [revised]
PHST- 2016/08/05 00:00 [accepted]
PHST- 2016/09/15 06:00 [entrez]
PHST- 2016/09/15 06:00 [pubmed]
PHST- 2017/11/14 06:00 [medline]
PHST- 2016/09/13 00:00 [pmc-release]
AID - S2211-1247(16)31070-1 [pii]
AID - 10.1016/j.celrep.2016.08.023 [doi]
PST - ppublish
SO  - Cell Rep. 2016 Sep 13;16(11):2953-2966. doi: 10.1016/j.celrep.2016.08.023.