PMID- 27627970
OWN - NLM
STAT- MEDLINE
DCOM- 20170127
LR  - 20170127
IS  - 0387-821X (Print)
IS  - 0387-821X (Linking)
VI  - 38
IP  - 3
DP  - 2016 Sep
TI  - Molecular Approaches to Studying Microbial Communities: Targeting the 16S 
      Ribosomal RNA Gene.
PG  - 223-32
LID - 10.7888/juoeh.38.223 [doi]
AB  - Culture-independent methods to detect microorganisms have been developed in 
      parallel with traditional culture-based methods ever since the classification of 
      bacteria based on 16S rRNA gene sequences was advocated in the 1970s. The 
      development and the prevalence of culture-independent molecular technologies have 
      provided revolutionary progress in microbial studies. The development of these 
      technologies contributes significantly to the research of microorganisms that 
      cannot be detected by traditional methods such as culture-dependent methods.Many 
      molecular methods targeting the 16S rRNA gene, such as fluorescence in situ 
      hybridization (FISH), quantitative PCR, terminal restriction fragment length 
      polymorphism (T-RFLP), denaturing-gradient gel electrophoresis (DGGE), clone 
      library analysis, and next-generation DNA sequencing (NGS) technologies, have 
      been applied to various microbial studies. Notably, the advent of NGS 
      technologies enabled a large-scale research of the bacterial community. Many 
      recent studies using the NGS technologies have revealed that a larger number of 
      bacteria and taxa than previously thought inhabit various parts of the human body 
      and various places on the earth. The principles and characteristics of each 
      molecular method are different, and each method possesses individual advantages; 
      for example target specificity, comprehensiveness, rapidness, and cost 
      efficiency. Therefore it is important that the methods used in studies are 
      suitable for the objective and materials. Herein, we highlights molecular 
      approaches targeting the 16S rRNA gene in bacterial community analysis, and 
      focuses on the advantages and limitations of each technology.
FAU - Fukuda, Kazumasa
AU  - Fukuda K
AD  - Department of Microbiology, School of Medicine, University of Occupational and 
      Environmental Health, Japan.
FAU - Ogawa, Midori
AU  - Ogawa M
FAU - Taniguchi, Hatsumi
AU  - Taniguchi H
FAU - Saito, Mitsumasa
AU  - Saito M
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - J UOEH
JT  - Journal of UOEH
JID - 7909645
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Bacterial/*genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Sequence Analysis, DNA/methods
EDAT- 2016/09/16 06:00
MHDA- 2017/01/28 06:00
CRDT- 2016/09/16 06:00
PHST- 2016/09/16 06:00 [entrez]
PHST- 2016/09/16 06:00 [pubmed]
PHST- 2017/01/28 06:00 [medline]
AID - 10.7888/juoeh.38.223 [doi]
PST - ppublish
SO  - J UOEH. 2016 Sep;38(3):223-32. doi: 10.7888/juoeh.38.223.