PMID- 27629909
OWN - NLM
STAT- MEDLINE
DCOM- 20170217
LR  - 20221207
IS  - 1758-1001 (Electronic)
IS  - 0004-5632 (Linking)
VI  - 54
IP  - 1
DP  - 2017 Jan
TI  - Identification of haemoglobin New York by haemoglobin A(1c) measurement using the 
      Sebia Capillarys 2 Flex Piercing system.
PG  - 178-182
LID - 10.1177/0004563216671756 [doi]
AB  - Haemoglobinopathies may interfere with the haemoglobin A(1c) (HbA(1c)) 
      measurement, leading to incorrect diagnosis and inappropriate treatment. It is 
      essential that HbA(1c) assays are capable of identifying haemoglobinopathies. We 
      report two cases of haemoglobin New York (HbNY) discovered through HbA(1c) 
      analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], 
      Sebia). We used these samples to evaluate the ability of three other HbA(1c) 
      assays to identify this variant: ion-exchange high-performance liquid 
      chromatography (Variant II Turbo [VII-T], Bio-Rad); boronate affinity 
      high-performance liquid chromatography (Ultra(2), Trinity Biotech) and 
      immunoassay (Cobas c501 Tina-quant Generation 3, Roche Diagnostics). Each method 
      was used for HbA(1c) assay of in samples from two cases of heterozygous 
      haemoglobinopathy: beta(0)-thalassemia/HbNY (Case 1) and HbA/NY (Case 2). Only the 
      C2FP system detected HbNY (an additional peak appeared between HbA(1c) and 
      HbA(0)). Clinical laboratories should be aware of the limitations of their 
      HbA(1c) assay methods especially in geographic areas, where haemoglobinopathy 
      prevalence is high.
FAU - Chao, Yan
AU  - Chao Y
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Wan, Zemin
AU  - Wan Z
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Wu, Xiaobin
AU  - Wu X
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Qiu, Feng
AU  - Qiu F
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Wu, Xinzhong
AU  - Wu X
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Wang, Yunxiu
AU  - Wang Y
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Ke, Peifeng
AU  - Ke P
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Xu, Jianhua
AU  - Xu J
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Zhuang, Junhua
AU  - Zhuang J
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
FAU - Huang, Xianzhang
AU  - Huang X
AD  - Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, China.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20161020
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Hemoglobins, Abnormal)
RN  - 0 (hemoglobin A1c protein, human)
RN  - 9035-15-8 (hemoglobin New York)
SB  - IM
MH  - Aged
MH  - Chromatography, Affinity
MH  - Chromatography, Ion Exchange
MH  - Electrophoresis, Capillary/*instrumentation
MH  - Glycated Hemoglobin/*isolation & purification
MH  - Hemoglobins, Abnormal/*isolation & purification
MH  - Humans
MH  - Male
MH  - Young Adult
MH  - beta-Thalassemia/blood/*diagnosis
OTO - NOTNLM
OT  - Haemoglobin A1c
OT  - boronate affinity high-performance liquid chromatography
OT  - capillary electrophoresis
OT  - haemoglobin New York
OT  - immunoassay
OT  - ion-exchange high-performance liquid chromatography
EDAT- 2016/09/16 06:00
MHDA- 2017/02/18 06:00
CRDT- 2016/09/16 06:00
PHST- 2016/09/16 06:00 [pubmed]
PHST- 2017/02/18 06:00 [medline]
PHST- 2016/09/16 06:00 [entrez]
AID - 0004563216671756 [pii]
AID - 10.1177/0004563216671756 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 2017 Jan;54(1):178-182. doi: 10.1177/0004563216671756. Epub 
      2016 Oct 20.