PMID- 27630655 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160916 LR - 20200928 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 7 DP - 2016 TI - The Effect of Green Synthesized CuO Nanoparticles on Callogenesis and Regeneration of Oryza sativa L. PG - 1330 LID - 10.3389/fpls.2016.01330 [doi] LID - 1330 AB - In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40 +/- 5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale. FAU - Anwaar, Sadaf AU - Anwaar S AD - Department of Biotechnology and Bioinformatics, International Islamic University Islamabad Islamabad, Pakistan. FAU - Maqbool, Qaisar AU - Maqbool Q AD - Department of Biotechnology, Virtual University of PakistanLahore, Pakistan; Interdisciplinary Research Organization, University of ChakwalChakwal, Pakistan. FAU - Jabeen, Nyla AU - Jabeen N AD - Department of Biotechnology and Bioinformatics, International Islamic University Islamabad Islamabad, Pakistan. FAU - Nazar, Mudassar AU - Nazar M AD - Department of Biotechnology, Virtual University of Pakistan Lahore, Pakistan. FAU - Abbas, Fazal AU - Abbas F AD - Interdisciplinary Research Organization, University of ChakwalChakwal, Pakistan; Department of Physics, International Islamic University IslamabadIslamabad, Pakistan. FAU - Nawaz, Bushra AU - Nawaz B AD - Department of Mechatronics, University of Engineering and Technology Taxila Chakwal, Pakistan. FAU - Hussain, Talib AU - Hussain T AD - National Institute of Vacuum Science and Technology Islamabad, Pakistan. FAU - Hussain, Syed Z AU - Hussain SZ AD - Department of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan. LA - eng PT - Journal Article DEP - 20160831 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 EIN - Front Plant Sci. 2020 May 06;11:540. PMID: 32435257 PMC - PMC5005396 OTO - NOTNLM OT - FTIR OT - SEM OT - XRD OT - callus induction OT - copper oxide nanoparticles OT - regeneration EDAT- 2016/09/16 06:00 MHDA- 2016/09/16 06:01 PMCR- 2016/01/01 CRDT- 2016/09/16 06:00 PHST- 2016/06/30 00:00 [received] PHST- 2016/08/18 00:00 [accepted] PHST- 2016/09/16 06:00 [entrez] PHST- 2016/09/16 06:00 [pubmed] PHST- 2016/09/16 06:01 [medline] PHST- 2016/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2016.01330 [doi] PST - epublish SO - Front Plant Sci. 2016 Aug 31;7:1330. doi: 10.3389/fpls.2016.01330. eCollection 2016.