PMID- 27630705
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160916
LR  - 20220408
IS  - 1673-5374 (Print)
IS  - 1876-7958 (Electronic)
IS  - 1673-5374 (Linking)
VI  - 11
IP  - 7
DP  - 2016 Jul
TI  - Extracellular matrix from human umbilical cord-derived mesenchymal stem cells as 
      a scaffold for peripheral nerve regeneration.
PG  - 1172-9
LID - 10.4103/1673-5374.187061 [doi]
AB  - The extracellular matrix, which includes collagens, laminin, or fibronectin, 
      plays an important role in peripheral nerve regeneration. Recently, a Schwann 
      cell-derived extracellular matrix with classical biomaterial was used to mimic 
      the neural niche. However, extensive clinical use of Schwann cells remains 
      limited because of the limited origin, loss of an autologous nerve, and extended 
      in vitro culture times. In the present study, human umbilical cord-derived 
      mesenchymal stem cells (hUCMSCs), which are easily accessible and more 
      proliferative than Schwann cells, were used to prepare an extracellular matrix. 
      We identified the morphology and function of hUCMSCs and investigated their 
      effect on peripheral nerve regeneration. Compared with a non-coated dish tissue 
      culture, the hUCMSC-derived extracellular matrix enhanced Schwann cell 
      proliferation, upregulated gene and protein expression levels of brain-derived 
      neurotrophic factor, glial cell-derived neurotrophic factor, and vascular 
      endothelial growth factor in Schwann cells, and enhanced neurite outgrowth from 
      dorsal root ganglion neurons. These findings suggest that the hUCMSC-derived 
      extracellular matrix promotes peripheral nerve repair and can be used as a basis 
      for the rational design of engineered neural niches.
FAU - Xiao, Bo
AU  - Xiao B
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China; The 
      Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu 
      Province, China.
FAU - Rao, Feng
AU  - Rao F
AD  - Department of Orthopedics, First Affiliated Hospital, School of Medicine, Shihezi 
      University, Shihezi, Xinjiang Uygur Autonomous Region, China.
FAU - Guo, Zhi-Yuan
AU  - Guo ZY
AD  - Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, 
      China.
FAU - Sun, Xun
AU  - Sun X
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Wang, Yi-Guo
AU  - Wang YG
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Liu, Shu-Yun
AU  - Liu SY
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Wang, Ai-Yuan
AU  - Wang AY
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Guo, Quan-Yi
AU  - Guo QY
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Meng, Hao-Ye
AU  - Meng HY
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The 
      Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu 
      Province, China.
FAU - Peng, Jiang
AU  - Peng J
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The 
      Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu 
      Province, China.
FAU - Wang, Yu
AU  - Wang Y
AUID- ORCID: 0000-0001-7811-5834
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The 
      Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu 
      Province, China.
FAU - Lu, Shi-Bi
AU  - Lu SB
AUID- ORCID: 0000-0001-5333-4061
AD  - Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing 
      Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Neural Regen Res
JT  - Neural regeneration research
JID - 101316351
PMC - PMC4994464
OTO - NOTNLM
OT  - Schwann cell
OT  - collagen
OT  - dorsal root ganglion neuron
OT  - extracellular matrix
OT  - fibronectin
OT  - laminin
OT  - mesenchymal stem cells
OT  - nerve regeneration
OT  - neural regeneration
OT  - neurotropic factors
OT  - niche
OT  - peripheral nerve regeneration
COIS- Conflicts of Interest: None declared.
EDAT- 2016/09/16 06:00
MHDA- 2016/09/16 06:01
PMCR- 2016/07/01
CRDT- 2016/09/16 06:00
PHST- 2016/09/16 06:00 [entrez]
PHST- 2016/09/16 06:00 [pubmed]
PHST- 2016/09/16 06:01 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - NRR-11-1172 [pii]
AID - 10.4103/1673-5374.187061 [doi]
PST - ppublish
SO  - Neural Regen Res. 2016 Jul;11(7):1172-9. doi: 10.4103/1673-5374.187061.