PMID- 27634482
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20180521
LR  - 20240104
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Sep 16
TI  - Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study.
PG  - 33537
LID - 10.1038/srep33537 [doi]
LID - 33537
AB  - Electroporation is a phenomenon occurring due to exposure of cells to Pulsed 
      Electric Fields (PEF) which leads to increase of membrane permeability. 
      Electroporation is used in medicine, biotechnology, and food processing. 
      Recently, as an alternative to electroporation by PEF, Pulsed ElectroMagnetic 
      Fields (PEMF) application causing similar biological effects was suggested. Since 
      induced electric field in PEMF however is 2-3 magnitudes lower than in PEF 
      electroporation, the membrane permeabilization mechanism remains hypothetical. We 
      have designed pilot experiments where Saccharomyces cerevisiae and Candida 
      lusitaniae cells were subjected to single 100-250 mus electrical pulse of 800 V 
      with and without concomitant delivery of magnetic pulse (3, 6 and 9 T). As 
      expected, after the PEF pulses only the number of Propidium Iodide (PI) 
      fluorescent cells has increased, indicative of membrane permeabilization. We 
      further show that single sub-millisecond magnetic field pulse did not cause 
      detectable poration of yeast. Concomitant exposure of cells to pulsed electric 
      (PEF) and magnetic field (PMF) however resulted in the increased number PI 
      fluorescent cells and reduced viability. Our results show increased membrane 
      permeability by PEF when combined with magnetic field pulse, which can explain 
      electroporation at considerably lower electric field strengths induced by PEMF 
      compared to classical electroporation.
FAU - Novickij, Vitalij
AU  - Novickij V
AD  - Vilnius Gediminas Technical University, Institute of High Magnetic Fields, 
      Vilnius, 03227, Lithuania.
FAU - Grainys, Audrius
AU  - Grainys A
AD  - Vilnius Gediminas Technical University, Institute of High Magnetic Fields, 
      Vilnius, 03227, Lithuania.
FAU - Lastauskiene, Egle
AU  - Lastauskiene E
AD  - Vilnius University, Department of Biotechnology and Microbiology, Vilnius, 03101, 
      Lithuania.
FAU - Kananaviciute, Ruta
AU  - Kananaviciute R
AD  - Vilnius University, Department of Biotechnology and Microbiology, Vilnius, 03101, 
      Lithuania.
FAU - Pamedytyte, Dovile
AU  - Pamedytyte D
AD  - Vilnius University, Department of Biotechnology and Microbiology, Vilnius, 03101, 
      Lithuania.
FAU - Kalediene, Lilija
AU  - Kalediene L
AD  - Vilnius University, Department of Biotechnology and Microbiology, Vilnius, 03101, 
      Lithuania.
FAU - Novickij, Jurij
AU  - Novickij J
AD  - Vilnius Gediminas Technical University, Institute of High Magnetic Fields, 
      Vilnius, 03227, Lithuania.
FAU - Miklavcic, Damijan
AU  - Miklavcic D
AD  - University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, SI-1000, 
      Slovenia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160916
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
PMC - PMC5025861
EDAT- 2016/09/17 06:00
MHDA- 2016/09/17 06:01
PMCR- 2016/09/16
CRDT- 2016/09/17 06:00
PHST- 2016/02/15 00:00 [received]
PHST- 2016/08/30 00:00 [accepted]
PHST- 2016/09/17 06:00 [entrez]
PHST- 2016/09/17 06:00 [pubmed]
PHST- 2016/09/17 06:01 [medline]
PHST- 2016/09/16 00:00 [pmc-release]
AID - srep33537 [pii]
AID - 10.1038/srep33537 [doi]
PST - epublish
SO  - Sci Rep. 2016 Sep 16;6:33537. doi: 10.1038/srep33537.