PMID- 27634771
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR  - 20171221
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 337
DP  - 2016 Nov 19
TI  - Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the 
      nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
PG  - 66-87
LID - S0306-4522(16)30442-0 [pii]
LID - 10.1016/j.neuroscience.2016.09.004 [doi]
AB  - Treatment strategies for ischemic stroke are still limited, since numerous 
      attempts were successful only in preclinical research but failed under clinical 
      condition. To overcome this translational roadblock, clinical relevant stroke 
      models should consider co-morbidities, age-related effects and the complex 
      neurovascular unit (NVU) concept. The NVU includes neurons, vessels and glial 
      cells with astrocytic endfeet in close relation to the extracellular matrix 
      (ECM). However, the role of the ECM after stroke-related tissue damage is poorly 
      understood and mostly neglected for treatment strategies. This study is focused 
      on alterations of perineuronal nets (PNs) as ECM constituents and 
      parvalbumin-containing GABAergic neurons in mice with emphasis on the nucleus 
      reticularis thalami (NRT) in close proximity to the ischemic lesion as induced by 
      a filament-based stroke model. One day after ischemia onset, 
      immunofluorescence-based quantitative analyses revealed drastically declined PNs 
      in the ischemia-affected NRT from 3- and 12-month-old wildtype and co-morbid 
      triple-transgenic (3xTg) mice with Alzheimer-like alterations. 
      Parvalbumin-positive cells decreased numerically in the ischemia-affected NRT, 
      while staining intensity did not differ between the affected and non-affected 
      hemisphere. Additional qualitative analyses demonstrated ischemia-induced loss of 
      PNs and allocated neuropil ECM immunoreactive for aggrecan and neurocan, and 
      impaired immunoreactivity for calbindin, the potassium channel subunit Kv3.1b and 
      the glutamate decarboxylase isoforms GAD65 and GAD67 in the NRT. In conclusion, 
      these data confirm PNs as highly sensitive constituents of the ECM along with 
      impaired neuronal integrity of GABAergic neurons. Therefore, specific targeting 
      of ECM components might appear as a promising strategy for future treatment 
      strategies in stroke.
CI  - Copyright (c) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Hartig, Wolfgang
AU  - Hartig W
AD  - Paul Flechsig Institute for Brain Research University of Leipzig, Liebigstr. 19, 
      04103 Leipzig, Germany. Electronic address: hartig@medizin.uni-leipzig.de.
FAU - Appel, Simon
AU  - Appel S
AD  - Paul Flechsig Institute for Brain Research University of Leipzig, Liebigstr. 19, 
      04103 Leipzig, Germany.
FAU - Suttkus, Anne
AU  - Suttkus A
AD  - Paul Flechsig Institute for Brain Research University of Leipzig, Liebigstr. 19, 
      04103 Leipzig, Germany; Department of Pediatric Surgery, University Hospital 
      Leipzig, Liebigstr. 20 A, 04103 Leipzig, Germany.
FAU - Grosche, Jens
AU  - Grosche J
AD  - Effigos GmbH, Am Deutschen Platz 4, 04103 Leipzig, Germany.
FAU - Michalski, Dominik
AU  - Michalski D
AD  - Department of Neurology, University of Leipzig, Liebigstr. 20, 04103 Leipzig, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20160912
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (Potassium Channels)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Immunohistochemistry/methods
MH  - Male
MH  - Mice, Transgenic
MH  - Nerve Net/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*metabolism
MH  - Parvalbumins/*metabolism
MH  - Pons/metabolism
MH  - Potassium Channels/*metabolism
MH  - Stroke/*metabolism/physiopathology
OTO - NOTNLM
OT  - animal model
OT  - cerebral ischemia
OT  - extracellular matrix
OT  - perineuronal net
OT  - reticular thalamic nucleus
OT  - triple-transgenic mouse
EDAT- 2016/10/25 06:00
MHDA- 2017/12/22 06:00
CRDT- 2016/09/17 06:00
PHST- 2016/05/30 00:00 [received]
PHST- 2016/08/18 00:00 [revised]
PHST- 2016/09/03 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
PHST- 2016/09/17 06:00 [entrez]
AID - S0306-4522(16)30442-0 [pii]
AID - 10.1016/j.neuroscience.2016.09.004 [doi]
PST - ppublish
SO  - Neuroscience. 2016 Nov 19;337:66-87. doi: 10.1016/j.neuroscience.2016.09.004. 
      Epub 2016 Sep 12.