PMID- 27636508
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20171218
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 78
DP  - 2016 Oct
TI  - Atractylone, an active constituent of KMP6, attenuates allergic inflammation on 
      allergic rhinitis in vitro and in vivo models.
PG  - 121-132
LID - S0161-5890(16)30180-8 [pii]
LID - 10.1016/j.molimm.2016.09.007 [doi]
AB  - KMP6 (Pyeongwee-San) is a Korean Medicine used to treat gastrointestinal 
      disorders. Recently, we reported KMP6 had beneficial effects on allergic 
      inflammatory diseases. The aim of this study was to evaluate the effects of 
      atractylone (Atr), a constituent of KMP6, on allergic rhinitis (AR) and to 
      identify the mechanism responsible for these effects. The anti-allergic 
      inflammatory effects of Atr were evaluated on phorbol 12-myristate 13-acetate and 
      calcium ionophore A23187 (PMACI)-stimulated human mast cell line, HMC-1 cells and 
      in an ovalbumin (OVA)-induced AR animal model using Western blotting, 
      quantitative real-time PCR, ELISA, and immunohistochemistry methods. In HMC-1 
      cells, Atr and KMP6 attenuated PMACI-caused proinflammatory cytokine production 
      and mRNA expression. We found that PMACI induced caspase-1/nuclear factor 
      (NF)-kappaB/mitogen activated protein kinases (MAPKs) activation. PMACI-caused 
      caspase-1/NF-kappaB/MAPKs activations were attenuated by Atr and KMP6. In AR animal 
      model, Atr and KMP6 reduced AR clinical symptoms and biomarkers including rub 
      scores, total IgE, histamine, prostaglandin D(2), thymic stromal lymphopoietin, 
      interleukin (IL)-1beta, IL-4, IL-5, IL-6, IL-13, tumor necrosis factor-alpha, 
      cyclooxygenase-2, intercellular adhesion molecule-1, and macrophage inflammatory 
      protein-2. In addition, Atr and KMP6 attenuated eosinophils and mast cells 
      invasions into nasal mucosa tissues and diminished mast cell-derived caspase-1 
      activation. These results indicate that Atr is an active constituent of KMP6 and 
      a potential therapeutic agent for AR.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Kim, Hee-Yun
AU  - Kim HY
AD  - Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 
      Seoul, 02447, Republic of Korea.
FAU - Nam, Sun-Young
AU  - Nam SY
AD  - Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 
      Seoul, 02447, Republic of Korea.
FAU - Hwang, Sung-Yeoun
AU  - Hwang SY
AD  - Korea Bio Medical Science Institute, Gangnam-gu, Seoul, 06106, Republic of Korea.
FAU - Kim, Hyung-Min
AU  - Kim HM
AD  - Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 
      Seoul, 02447, Republic of Korea. Electronic address: hmkim@khu.ac.kr.
FAU - Jeong, Hyun-Ja
AU  - Jeong HJ
AD  - Department of Food Technology and Inflammatory Disease Research Center, Hoseo 
      University, Asan, Chungnam, 31499, Republic of Korea. Electronic address: 
      hjjeong@hoseo.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160913
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Plant Extracts)
RN  - 0 (Sesquiterpenes)
RN  - 0 (pyeongwee-san extract)
RN  - 6989-21-5 (atractylon)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chromatography, Liquid
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation/immunology
MH  - Mast Cells/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nasal Mucosa/drug effects
MH  - Plant Extracts/pharmacology
MH  - Real-Time Polymerase Chain Reaction
MH  - Rhinitis, Allergic/*immunology
MH  - Sesquiterpenes/*pharmacology
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Allergic rhinitis
OT  - Atractylone
OT  - Caspase-1
OT  - Mast cells
OT  - Proinflammatory cytokine
OT  - Thymic stromal lymphopoietin
EDAT- 2016/09/17 06:00
MHDA- 2017/09/16 06:00
CRDT- 2016/09/17 06:00
PHST- 2016/06/06 00:00 [received]
PHST- 2016/09/07 00:00 [revised]
PHST- 2016/09/08 00:00 [accepted]
PHST- 2016/09/17 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
PHST- 2016/09/17 06:00 [entrez]
AID - S0161-5890(16)30180-8 [pii]
AID - 10.1016/j.molimm.2016.09.007 [doi]
PST - ppublish
SO  - Mol Immunol. 2016 Oct;78:121-132. doi: 10.1016/j.molimm.2016.09.007. Epub 2016 
      Sep 13.