PMID- 27638307
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20181202
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 479
IP  - 2
DP  - 2016 Oct 14
TI  - EZH2 promotes invasion and metastasis of laryngeal squamous cells carcinoma via 
      epithelial-mesenchymal transition through H3K27me3.
PG  - 253-259
LID - S0006-291X(16)31515-7 [pii]
LID - 10.1016/j.bbrc.2016.09.055 [doi]
AB  - Enhancer of Zeste Homolog 2(EZH2), which can change chromatin structure by 
      tri-methylation of the 27th lysine of H3 in nucleosome histone (H3K27me3), is 
      involved in different types of cancers. However, the role and mechanism 
      underlying aberrant EZH2 expression in laryngeal squamous cells carcinoma (LSCC) 
      remain unclear. In the present study, we found that down-regulation of EZH2 and 
      H3K27me3 in LSCC cells (Hep-2 and SCC10A) resulted in an mesenchymal-epithelial 
      transition(MET) like cell morphology and lower invasion in vitro, weakened tumor 
      growth, intrahepatic and pulmonary metastasis in vivo. Furthermore, EZH2 promoted 
      the epithelial-mesenchymal transition(EMT) process through down-regulation of 
      Ca(2+) dependent cell adhesion molecule E (E-cadherin) and up-regulation of 
      H3K27me3 in vitro and in vivo. Moreover, E-cadherin was transcriptionally induced 
      upon stable knockdown of EZH2, and quantitative chromatin 
      immunoprecipitation(qChIP) analysis confirmed the depletion of H3K27me3 
      enrichment on E-cadherin promoter upon EZH2 knockdown in Hep-2 and SCC10A cells. 
      In addition, the expression of EZH2 was positively correlated with that of 
      H3K27me3 and both of them were inversely correlated with E-cadherin expression in 
      human LSCC tissues. In summary, this study indicated that EZH2 promoted invasion 
      and metastasis of LSCC via EMT through H3K27me3.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Luo, HuaNan
AU  - Luo H
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Jiang, Yuan
AU  - Jiang Y
AD  - Department of Otolaryngology-Head & Neck Surgery, Xi'an Xd Group Hospital, Feng 
      Deng Road #97, Xi'an 710077, Shan'xi Province, China.
FAU - Ma, SiJing
AU  - Ma S
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Chang, HuanHuan
AU  - Chang H
AD  - Department of Otolaryngology-Head & Neck Surgery, Xi'an Children's Hospital, West 
      of the Hospital Lane #69, Xi'an 710003, Shan'xi Province, China.
FAU - Yi, ChunXi
AU  - Yi C
AD  - Department of Otolaryngology-Head & Neck Surgery, Xi'an Center Hospital, Xi Wu 
      Road #161, Xi'an 710004, Shan'xi Province, China.
FAU - Cao, Hui
AU  - Cao H
AD  - Department of Otolaryngology-Head & Neck Surgery, The First Affiliated Hospital 
      of Xi'an Medical University, Feng Gao Road #69, Xi'an 710077, Shan'xi Province, 
      China.
FAU - Gao, Ying
AU  - Gao Y
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Guo, HaiLi
AU  - Guo H
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Hou, Jin
AU  - Hou J
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Yan, Jing
AU  - Yan J
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Sheng, Ying
AU  - Sheng Y
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China.
FAU - Ren, XiaoYong
AU  - Ren X
AD  - Department of Otolaryngology-Head & Neck Surgery, The Second Affiliated Hospital 
      of Xi'an Jiao Tong University, Xi Wu Road #157, Xi'an 710004, Shan'xi Province, 
      China. Electronic address: renxiaoyong@vip.126.com.
LA  - eng
PT  - Journal Article
DEP - 20160913
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Histones)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antigens, CD
MH  - Cadherins/metabolism
MH  - Calcium/metabolism
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cell Line, Tumor
MH  - Enhancer of Zeste Homolog 2 Protein/*metabolism
MH  - Epithelial-Mesenchymal Transition
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Histones/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*metabolism
MH  - Lymphatic Metastasis
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Promoter Regions, Genetic
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - EZH2
OT  - Epithelial-mesenchymal transition
OT  - H3K27me3
OT  - Laryngeal squamous cells carcinoma
OT  - Metastasis
EDAT- 2016/09/18 06:00
MHDA- 2017/06/07 06:00
CRDT- 2016/09/18 06:00
PHST- 2016/09/09 00:00 [received]
PHST- 2016/09/12 00:00 [accepted]
PHST- 2016/09/18 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
PHST- 2016/09/18 06:00 [entrez]
AID - S0006-291X(16)31515-7 [pii]
AID - 10.1016/j.bbrc.2016.09.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Oct 14;479(2):253-259. doi: 
      10.1016/j.bbrc.2016.09.055. Epub 2016 Sep 13.