PMID- 27640887
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20170208
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 165
DP  - 2016 Nov 15
TI  - Comparing the effects of endogenous and synthetic cannabinoid receptor agonists 
      on survival of gastric cancer cells.
PG  - 56-62
LID - S0024-3205(16)30567-7 [pii]
LID - 10.1016/j.lfs.2016.09.010 [doi]
AB  - AIMS: Anti-neoplastic activity induced by cannabinoids has been extensively 
      documented for a number of cancer cell types; however, this topic has been 
      explored in gastric cancer cells only in a limited number of approaches. Thus, 
      the need of integrative and comparative studies still persists. MATERIALS AND 
      METHODS: In this study we tested and compared the effects of three different 
      cannabinoid receptor agonists-anandamide (AEA), (R)-(+)-methanandamide (Meth-AEA) 
      and CP 55,940 (CP)- on gastric cancer cell morphology, viability and death events 
      in order to provide new insights to the use of these agents for therapeutic 
      purposes. KEY FINDINGS: The three agents tested exhibited similar 
      concentration-dependent effects in the induction of changes in cell morphology 
      and cell loss, as well as in the decrease of cell viability and DNA laddering in 
      the human gastric adenocarcinoma cell line (AGS). Differences among the 
      cannabinoids tested were mostly observed in the density of cells found in early 
      and late apoptosis and necrosis, favoring AEA and CP as the more effective 
      inducers of apoptotic mechanisms, and Meth-AEA as a more effective inducer of 
      necrosis through transient and rapid apoptosis. SIGNIFICANCE: Through a 
      comparative approach, our results support and confirm the therapeutic potential 
      that cannabinoid receptor agonists exert in gastric cancer cells and open 
      possibilities to use cannabinoids as part of a new gastric cancer therapy.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Ortega, A
AU  - Ortega A
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Garcia-Hernandez, V M
AU  - Garcia-Hernandez VM
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Ruiz-Garcia, E
AU  - Ruiz-Garcia E
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Meneses-Garcia, A
AU  - Meneses-Garcia A
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Herrera-Gomez, A
AU  - Herrera-Gomez A
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Aguilar-Ponce, J L
AU  - Aguilar-Ponce JL
AD  - Laboratorio de Medicina Translacional, Instituto Nacional de Cancerologia, SSA. 
      Mexico City 14080, Mexico.
FAU - Montes-Servin, E
AU  - Montes-Servin E
AD  - Unidad de Oncologia Toracica, Instituto Nacional de Cancerologia, SSA. Mexico 
      City 14080, Mexico.
FAU - Prospero-Garcia, O
AU  - Prospero-Garcia O
AD  - Laboratorio de Cannabinoides, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional Autonoma de Mexico City, 04510, Mexico.
FAU - Del Angel, S A
AU  - Del Angel SA
AD  - Laboratorio de Aminoacidos Excitadores, Instituto Nacional de Neurologia y 
      Neurocirugia, SSA. Mexico City 14269, Mexico. Electronic address: 
      absada@yahoo.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160915
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cannabinoid Receptor Agonists)
SB  - IM
MH  - Cannabinoid Receptor Agonists/*pharmacology
MH  - Cell Line, Tumor
MH  - Cell Survival/*drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - Stomach Neoplasms/*pathology
OTO - NOTNLM
OT  - Anti-proliferative activity
OT  - Anti-tumor therapy
OT  - Apoptosis
OT  - Cannabinoid receptor agonists
OT  - Gastric cancer cells
EDAT- 2016/10/23 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/09/20 06:00
PHST- 2016/08/11 00:00 [received]
PHST- 2016/09/09 00:00 [revised]
PHST- 2016/09/14 00:00 [accepted]
PHST- 2016/10/23 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
PHST- 2016/09/20 06:00 [entrez]
AID - S0024-3205(16)30567-7 [pii]
AID - 10.1016/j.lfs.2016.09.010 [doi]
PST - ppublish
SO  - Life Sci. 2016 Nov 15;165:56-62. doi: 10.1016/j.lfs.2016.09.010. Epub 2016 Sep 
      15.