PMID- 27643992
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20190212
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 9
DP  - 2016
TI  - Disruption of Prostate Microvasculature by Combining Microbubble-Enhanced 
      Ultrasound and Prothrombin.
PG  - e0162398
LID - 10.1371/journal.pone.0162398 [doi]
LID - e0162398
AB  - Previous studies have shown a unique method to disrupt tumor vasculature using 
      pulsed, high-pressure amplitude therapeutic ultrasound combined with 
      microbubbles. In this study, we attempted to destroy the prostate vasculature of 
      canine prostates using microbubble-enhanced ultrasound (MEUS) and prothrombin. 
      The prostates of 43 male mongrel canines were surgically exposed. Twenty-two 
      prostates were treated using MEUS (n = 11) or MEUS and prothrombin (PMEUS, n = 
      11). The other 21 prostates, which were treated using microbubbles (n = 7), 
      ultrasound (n = 7) or prothrombin (n = 7) only, served as the controls. 
      Prothrombin was intravenously infused at 20 IU/kg. MEUS was induced using a 
      therapeutic ultrasound device at a peak negative pressure of 4.47 MPa and a 
      microbubble injection. Contrast-enhanced ultrasound was performed to assess the 
      blood perfusion of the prostates. Then, the prostate tissue was harvested 
      immediately after treatment and at 48 hours later for pathological examination. 
      The contrast-enhanced ultrasound peak value of the prostate decreased 
      significantly from 36.2 +/- 5.6 to 27.1 +/- 6.3 after treatment in the PMEUS group, 
      but it remained unchanged in the other groups. Histological examination found 
      severe microvascular rupture, hemorrhage and thrombosis in both MEUS- and 
      PMEUS-treated prostates immediately after treatment, while disruption in the 
      PMEUS group was more severe than in the MEUS group. Forty-eight hours after 
      treatment, massive necrosis and infiltration of white blood cells occurred in the 
      PMEUS group. This study demonstrated that PMEUS disrupted the normal 
      microvasculature of canine prostates and induced massive necrosis. PMEUS could 
      potentially become a new noninvasive method used for the treatment of benign 
      prostatic hyperplasia.
FAU - Zhang, Jinlong
AU  - Zhang J
AD  - Department of Radiology, Research Institute of Surgery, Daping Hospital, Third 
      Military Medical University, Chongqing, China.
FAU - Wu, Shengzheng
AU  - Wu S
AD  - Department of Ultrasound, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, China.
FAU - Liu, Yongliang
AU  - Liu Y
AD  - Department of Urology, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, China.
FAU - Qiao, Lu
AU  - Qiao L
AD  - Department of Ultrasound, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, China.
FAU - Gao, Wenhong
AU  - Gao W
AD  - Department of Ultrasound, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, China.
FAU - Zhang, Weiguo
AU  - Zhang W
AD  - Department of Radiology, Research Institute of Surgery, Daping Hospital, Third 
      Military Medical University, Chongqing, China.
AD  - State key laboratory of Trauma, Burns and Combined Injury, Research Institute of 
      Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
FAU - Liu, Zheng
AU  - Liu Z
AD  - Department of Ultrasound, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20160919
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 9001-26-7 (Prothrombin)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - High-Intensity Focused Ultrasound Ablation/*methods
MH  - Male
MH  - Microbubbles/*therapeutic use
MH  - Microvessels/*pathology
MH  - Prostate/*blood supply/pathology
MH  - Prostatic Hyperplasia/pathology/*therapy
MH  - Prothrombin/administration & dosage/*therapeutic use
PMC - PMC5028116
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/09/20 06:00
MHDA- 2017/08/15 06:00
PMCR- 2016/09/19
CRDT- 2016/09/20 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/09/20 06:00 [entrez]
PHST- 2016/09/20 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
PHST- 2016/09/19 00:00 [pmc-release]
AID - PONE-D-16-10630 [pii]
AID - 10.1371/journal.pone.0162398 [doi]
PST - epublish
SO  - PLoS One. 2016 Sep 19;11(9):e0162398. doi: 10.1371/journal.pone.0162398. 
      eCollection 2016.