PMID- 27645905
OWN - NLM
STAT- MEDLINE
DCOM- 20170322
LR  - 20170923
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 58
DP  - 2016 Nov
TI  - Identification of Langerhans-like cells in the immunocompetent tissues of channel 
      catfish, Ictalurus punctatus.
PG  - 253-258
LID - S1050-4648(16)30586-1 [pii]
LID - 10.1016/j.fsi.2016.09.033 [doi]
AB  - Dendritic cells (DCs) are the most powerful antigen presenting cells (APCs) that 
      have a critical role in bridging innate and adaptive immune responses in 
      vertebrates. Dendritic cells have been characterized morphologically and 
      functionally in the teleost fish models such as rainbow trout, salmonids, medaka, 
      and zebrafish. The presence of DCs with remarkable similarities to human 
      Langerhans cells (LCs) has been described in the spleen and anterior kidney of 
      salmonids and rainbow trout. However, there is no evidence of the presence of DCs 
      and their role in channel catfish immunity. In this study, we assessed DC-like 
      cells in the immunocompetent tissues of channel catfish by immunohistochemistry 
      (IHC), flow cytometry and transmission electron microscopy (TEM). We identified 
      Langerin/CD207(+) (L/CD207(+)) cells in the channel catfish anterior kidney, 
      spleen and gill by IHC. Moreover, we described the cells that resembled mammal LC 
      DCs containing Birbeck-like (BL) granules in channel catfish spleen, anterior and 
      posterior kidneys and gill by TEM. Our data suggest that cells with DC-like 
      morphology in the immune related organs of catfish may share morphological and 
      functional properties with previously reported DCs in teleost fish and mammals. 
      More detailed knowledge of the phenotype and the function of catfish DCs will not 
      only help gain insight into the evolution of the vertebrate adaptive immune 
      system but will also provide valuable information for development and 
      optimization of immunotherapies and vaccination protocols for aquaculture use.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Kordon, Adef O
AU  - Kordon AO
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA.
FAU - Scott, Matthew A
AU  - Scott MA
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA.
FAU - Ibrahim, Iman
AU  - Ibrahim I
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA; Department of Pathology, Faculty of Veterinary 
      Medicine, Mansoura University, Mansoura, Egypt.
FAU - Abdelhamed, Hossam
AU  - Abdelhamed H
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA.
FAU - Ahmed, Hamada
AU  - Ahmed H
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA; Department of Nutrition and Veterinary Clinical 
      Nutrition, Faculty of Veterinary Medicine, Damanhour University, Egypt.
FAU - Baumgartner, Wes
AU  - Baumgartner W
AD  - Department of Pathobiology and Population Medicine, Collage of Veterinary 
      Medicine, Mississippi State University, MS 39762, USA.
FAU - Karsi, Attila
AU  - Karsi A
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA.
FAU - Pinchuk, Lesya M
AU  - Pinchuk LM
AD  - Department of Basic Sciences, College of Veterinary Medicine, Mississippi State 
      University, MS 39762, USA. Electronic address: pinchuk@cvm.msstate.edu.
LA  - eng
PT  - Journal Article
DEP - 20160916
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Animals
MH  - Flow Cytometry/veterinary
MH  - Gills/cytology/immunology/ultrastructure
MH  - Ictaluridae/*anatomy & histology/immunology
MH  - Immunohistochemistry/veterinary
MH  - Kidney/cytology/immunology/ultrastructure
MH  - Langerhans Cells/*cytology/ultrastructure
MH  - Microscopy, Electron, Transmission/veterinary
MH  - Spleen/cytology/immunology/ultrastructure
OTO - NOTNLM
OT  - Birbeck granules
OT  - Channel catfish
OT  - Dendritic cells
OT  - Langerhans cells
EDAT- 2016/10/23 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/09/21 06:00
PHST- 2016/06/15 00:00 [received]
PHST- 2016/08/19 00:00 [revised]
PHST- 2016/09/15 00:00 [accepted]
PHST- 2016/10/23 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
PHST- 2016/09/21 06:00 [entrez]
AID - S1050-4648(16)30586-1 [pii]
AID - 10.1016/j.fsi.2016.09.033 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Nov;58:253-258. doi: 10.1016/j.fsi.2016.09.033. Epub 
      2016 Sep 16.