PMID- 27647881
OWN - NLM
STAT- MEDLINE
DCOM- 20180126
LR  - 20240210
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 113
IP  - 40
DP  - 2016 Oct 4
TI  - WASH drives early recycling from macropinosomes and phagosomes to maintain 
      surface phagocytic receptors.
PG  - E5906-E5915
AB  - Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients 
      from extracellular media, which also allows immune cells to sample antigens from 
      their surroundings. During macropinosome formation, bulk plasma membrane is 
      internalized with all its integral proteins. It is vital for cells to salvage 
      these proteins before degradation, but the mechanisms for sorting them are not 
      known. Here we describe the evolutionarily conserved recruitment of the WASH 
      (WASP and SCAR homolog) complex to both macropinosomes and phagosomes within a 
      minute of internalization. Using Dictyostelium, we demonstrate that WASH drives 
      protein sorting and recycling from macropinosomes and is thus essential to 
      maintain surface receptor levels and sustain phagocytosis. WASH functionally 
      interacts with the retromer complex at both early and late phases of 
      macropinosome maturation, but mediates recycling via retromer-dependent and 
      -independent pathways. WASH mutants consequently have decreased membrane levels 
      of integrins and other surface proteins. This study reveals an important pathway 
      enabling cells to sustain macropinocytosis without bulk degradation of plasma 
      membrane components.
FAU - Buckley, Catherine M
AU  - Buckley CM
AD  - Department of Biomedical Sciences, Centre for Membrane Interactions and Dynamics, 
      University of Sheffield, Sheffield S10 2TN, United Kingdom; Bateson Centre, 
      University of Sheffield, Sheffield S10 2TN, United Kingdom.
FAU - Gopaldass, Navin
AU  - Gopaldass N
AD  - Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
FAU - Bosmani, Cristina
AU  - Bosmani C
AD  - Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
FAU - Johnston, Simon A
AU  - Johnston SA
AD  - Bateson Centre, University of Sheffield, Sheffield S10 2TN, United Kingdom; 
      Department of Infection, Immunity and Cardiovascular Sciences, University of 
      Sheffield Medical School, Sheffield S10 2RX, United Kingdom.
FAU - Soldati, Thierry
AU  - Soldati T
AUID- ORCID: 0000-0002-2056-7931
AD  - Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
FAU - Insall, Robert H
AU  - Insall RH
AD  - Beatson Institute for Cancer Research, Glasgow G61 1BD, United Kingdom 
      jason.king@sheffield.ac.uk r.insall@beatson.gla.ac.uk.
FAU - King, Jason S
AU  - King JS
AUID- ORCID: 0000-0003-0596-4506
AD  - Department of Biomedical Sciences, Centre for Membrane Interactions and Dynamics, 
      University of Sheffield, Sheffield S10 2TN, United Kingdom; Bateson Centre, 
      University of Sheffield, Sheffield S10 2TN, United Kingdom; 
      jason.king@sheffield.ac.uk r.insall@beatson.gla.ac.uk.
LA  - eng
GR  - 15672/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160919
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Integrins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Vesicular Transport Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - Cell Membrane/*metabolism
MH  - Dictyostelium/*metabolism
MH  - Golgi Apparatus/metabolism
MH  - Green Fluorescent Proteins/metabolism
MH  - Integrins/metabolism
MH  - Lysosomes/metabolism
MH  - Models, Biological
MH  - *Phagocytosis
MH  - Phagosomes/*metabolism
MH  - Protein Binding
MH  - Protein Transport
MH  - Receptors, Cell Surface/*metabolism
MH  - Vacuolar Proton-Translocating ATPases/metabolism
MH  - Vesicular Transport Proteins/*metabolism
PMC - PMC5056073
OTO - NOTNLM
OT  - Dictyostelium
OT  - WASH
OT  - macropinocytosis
OT  - phagocytosis
OT  - trafficking
COIS- The authors declare no conflict of interest.
EDAT- 2016/09/21 06:00
MHDA- 2018/01/27 06:00
PMCR- 2016/09/19
CRDT- 2016/09/21 06:00
PHST- 2016/09/21 06:00 [pubmed]
PHST- 2018/01/27 06:00 [medline]
PHST- 2016/09/21 06:00 [entrez]
PHST- 2016/09/19 00:00 [pmc-release]
AID - 1524532113 [pii]
AID - 201524532 [pii]
AID - 10.1073/pnas.1524532113 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5906-E5915. doi: 
      10.1073/pnas.1524532113. Epub 2016 Sep 19.