PMID- 27648128
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160920
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 8
IP  - 8
DP  - 2016
TI  - An experimental study on use of 7T MRI for evaluation of myocardial infarction in 
      SD rats transfected with pcDNA 3.1(+)/VEGF121 plasmid.
PG  - 3376-86
AB  - This study aims to build the myocardial infarction model in SD rats transfected 
      with pcDNA 3.1(+)/VEGF121 plasmid and study the effect of the transfection using 
      7T MRI. Twenty-four male SD rats were randomly divided into 2 groups, pcDNA 
      3.1(+)/VEGF121 plasmid transfection group (with improved coronary perfusion 
      delivery) and myocardial infarction model group. Cardiac cine magnetic resonance 
      imaging (Cine-MRI), T2-mapping and late gadolinium enhancement (LGE) cardiac 
      imaging were performed at 24 h, 48 h, 72 h and 7 d after myocardial infarction, 
      respectively. The signal intensity, area at risk (AAR), myocardium infarction 
      core (MIC) and salvageable myocardial zone (SMZ) were compared. The hearts were 
      harvested for anatomic characterization, which was related to pathological 
      examination (TTC staining, HE staining, Masson staining and immunohistochemical 
      staining). The Cine-MRI results showed that pcDNA 3.1(+)/VEGF121 plasmid 
      transfection group had higher end-diastolic volume (EDV) with a reduction in MIC 
      and SMZ, as compared with the myocardial infarction model group. MIC, SMZ and AAR 
      of the plasmid transfection declined over time. At 7 d, the two groups did not 
      differ significantly in AAR and T2 value. According to Western Blotting, VEGF was 
      up-regulated, while CaSR and caspase-3 were downregulated in the plasmid 
      transfection group, as compared with the model group. In conclusion, a good 
      treatment effect was achieved by coronary perfusion of pcDNA 3.1(+)/VEGF121 
      plasmid. 7T CMR sequences provide a non-invasive quantification of the treatment 
      efficacy. However, the assessment of myocardial injury using T2 value and AAR in 
      the presence of edema is less accurate. The myocardial protection of the plasmid 
      transfection group may be related to the inhibition of myocardial apoptosis, 
      vascular endothelial cell (VEC) proliferation and collagen proliferation. The 
      CaSR signaling pathway may contribute to reversing the apoptosis.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Radiology General Hospital of PLABeijing 100853, PR China; 
      Department of Radiology, Affiliated Hospital of Guizhou Medical 
      UniversityGuiyang, PR China.
FAU - Tian, Ruiqing
AU  - Tian R
AD  - Department of Oncology, The First People's Hospital of Guiyang PR China.
FAU - Shen, Xiangchun
AU  - Shen X
AD  - The Key Laboratory of Optional Utilization of Natural Medical Resource, Guizhou 
      Medical University University Town, Guian New District, Guiyang, PR China.
FAU - Chen, Yushu
AU  - Chen Y
AD  - Molecular Imaging Laboratory, Department of Radiology, West China Hospital, 
      Sichuan University PR China.
FAU - Chen, Wei
AU  - Chen W
AD  - Molecular Imaging Laboratory, Department of Radiology, West China Hospital, 
      Sichuan University PR China.
FAU - Gan, Lu
AU  - Gan L
AD  - Department of Radiology General Hospital of PLA Beijing 100853, PR China.
FAU - Shen, Guiquan
AU  - Shen G
AD  - Department of Radiology, Affiliated Hospital of Guizhou Medical University 
      Guiyang, PR China.
FAU - Ju, Haiyue
AU  - Ju H
AD  - Department of Radiology General Hospital of PLA Beijing 100853, PR China.
FAU - Yang, Li
AU  - Yang L
AD  - Department of Radiology General Hospital of PLA Beijing 100853, PR China.
FAU - Gao, Fabao
AU  - Gao F
AD  - Molecular Imaging Laboratory, Department of Radiology, West China Hospital, 
      Sichuan University PR China.
LA  - eng
PT  - Journal Article
DEP - 20160815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5009390
OTO - NOTNLM
OT  - CaSR
OT  - Gene therapy
OT  - myocardial infarction
OT  - salvageable myocardial zone
EDAT- 2016/09/21 06:00
MHDA- 2016/09/21 06:01
PMCR- 2016/08/15
CRDT- 2016/09/21 06:00
PHST- 2016/03/30 00:00 [received]
PHST- 2016/05/20 00:00 [accepted]
PHST- 2016/09/21 06:00 [entrez]
PHST- 2016/09/21 06:00 [pubmed]
PHST- 2016/09/21 06:01 [medline]
PHST- 2016/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2016 Aug 15;8(8):3376-86. eCollection 2016.