PMID- 27648133
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160920
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 8
IP  - 8
DP  - 2016
TI  - Condition medium of HepG-2 cells induces the transdifferentiation of human 
      umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells.
PG  - 3429-38
AB  - This study aimed to investigate the transdifferentiation of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) into cancer-associated mesenchymal stem cells 
      (CA-MSCs) after incubation with condition medium (CM) from liver cancer HepG-2 
      cells, and the biobehaviors (proliferation and migration) of these CA-MSCs were 
      further evaluated. The supernatant of HepG-2 cells was collected and mixed with 
      equal volume of low glucose DMEM. The resultant medium was used to treat hUCMSCs 
      for 48 h. The expression of CA-MSCs related proteins and miR-221 was detected in 
      cells. The supernatant of induced hUCMSCs was mixed with equal volume of high 
      glucose DMEM, and the resultant medium was used treat HepG-2 cells for 48 h and 
      the proliferation and migration of HepG-2 cells were evaluated. Moreover, HepG-2 
      cells were co-cultured with hUCMSCs and then the proliferation and migration of 
      HepG-2 cells were assessed. After incubation with the supernatant from HepG-2 
      cells, hUCMSCs showed significantly elevated expression of vimentin, fibroblast 
      activation protein (FAP) and miR-221. The supernatant of induced hUCMSCs was able 
      to significantly increase the proliferation and migration of HepG-2 cells. 
      Following co-culture, the proliferation and migration of HepG-2 cells increased 
      dramatically. These findings suggest that the supernatant of HepG-2 cells is able 
      to induce the phenotype of CA-MSCs and the supernatant of CA-MSCs may promote the 
      proliferation and migration of HepG-2 cells. These findings provide experimental 
      evidence for the cellular remodeling in tumor microenvironment and the safety of 
      clinical use of hUCMSCs.
FAU - Yang, Juan
AU  - Yang J
AD  - Department of Gastroenterology, The Third People's Hospital of Yunnan Province 
      Kunming 650011, China.
FAU - Miao, Yinglei
AU  - Miao Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University Kunming 650032, China.
FAU - Chang, Yefei
AU  - Chang Y
AD  - Department of Laboratory, The Third People's Hospital of Yunnan Province Kunming 
      650000, China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Department of Gastroenterology, The Third People's Hospital of Yunnan Province 
      Kunming 650011, China.
FAU - Wang, Yubo
AU  - Wang Y
AD  - Department of Gastroenterology, The Third People's Hospital of Yunnan Province 
      Kunming 650011, China.
FAU - Zheng, Sheng
AU  - Zheng S
AD  - Department of Gastroenterology, The Third People's Hospital of Yunnan Province 
      Kunming 650011, China.
LA  - eng
PT  - Journal Article
DEP - 20160815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5009395
OTO - NOTNLM
OT  - Stem cells
OT  - cell migration
OT  - hepatocellular carcinoma
OT  - transdifferentiation
OT  - umbilical cord mesenchymal stem cells
EDAT- 2016/09/21 06:00
MHDA- 2016/09/21 06:01
PMCR- 2016/08/15
CRDT- 2016/09/21 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/07/17 00:00 [accepted]
PHST- 2016/09/21 06:00 [entrez]
PHST- 2016/09/21 06:00 [pubmed]
PHST- 2016/09/21 06:01 [medline]
PHST- 2016/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2016 Aug 15;8(8):3429-38. eCollection 2016.