PMID- 27648960
OWN - NLM
STAT- MEDLINE
DCOM- 20170630
LR  - 20220331
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 101
IP  - 11
DP  - 2016 Nov
TI  - Role of Gut Microbiota and Short Chain Fatty Acids in Modulating Energy Harvest 
      and Fat Partitioning in Youth.
PG  - 4367-4376
AB  - OBJECTIVE: We aimed at determining the relationship of the gut microbiota and 
      short chain fatty acids with obesity and fat partitioning and at testing 
      potential differences in the ability of gut microbiota to ferment equal amounts 
      of carbohydrates (CHO) between lean and obese youth. RESEARCH DESIGN AND METHODS: 
      We analyzed the gut microbiota of 84 youth in whom body fat distribution was 
      measured by fast-magnetic resonance imaging, de novo lipogenesis (DNL) 
      quantitated using deuterated water, and the capability of gut flora to ferment 
      CHO was assessed by (13)C-fructose treatment in vitro. RESULTS: A significant 
      association was found between the Firmicutes to Bacteroidetes ratio, and the 
      abundance of Bacteroidetes and Actinobacteria with body mass index, visceral and 
      SC fat (all P < .05). Plasma acetate, propionate, and butyrate were associated 
      with body mass index and visceral and SC fat (all P < .05) and with hepatic DNL 
      (P = .01, P = .09, P = .04, respectively). Moreover, the rate of CHO fermentation 
      from the gut flora was higher in obese than in lean subjects (P = .018). 
      CONCLUSIONS: These data demonstrate that obese youth show a different gut flora 
      composition than lean and that short chain fatty acids are associated with body 
      fat partitioning and DNL. Also, the gut microbiota of obese youth have a higher 
      capability than the gut flora of lean to oxidize CHO.
FAU - Goffredo, Martina
AU  - Goffredo M
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Mass, Kendra
AU  - Mass K
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Parks, Elizabeth J
AU  - Parks EJ
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Wagner, David A
AU  - Wagner DA
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - McClure, Emily Ann
AU  - McClure EA
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Graf, Joerg
AU  - Graf J
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Savoye, Mary
AU  - Savoye M
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Pierpont, Bridget
AU  - Pierpont B
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Cline, Gary
AU  - Cline G
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
FAU - Santoro, Nicola
AU  - Santoro N
AD  - Department of Pediatrics (M.G., M.S., B.P., N.S.), Yale University, New Haven, 
      Connecticut; Universita' Milano Bicocca (M.G.), Milano, Italy; Department of 
      Molecular & Cell Biology (K.M., E.A.M., J.G.), University of Connecticut, Storrs, 
      Connecticut; Department of Medicine (E.J.P.), University of Missouri, Columbia, 
      Missouri; Metabolic Solutions Inc. (D.A.W.), Nashua, New Hampshire; Department of 
      Internal Medicine (G.C.), Yale University, New Haven, Connecticut.
LA  - eng
GR  - P30 DK045735/DK/NIDDK NIH HHS/United States
GR  - UL1 TR001863/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160920
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Fatty Acids, Volatile)
SB  - IM
MH  - Abdominal Fat/*metabolism
MH  - Actinobacteria/isolation & purification/*metabolism
MH  - Adolescent
MH  - Bacteroidetes/isolation & purification/*metabolism
MH  - *Body Mass Index
MH  - Child
MH  - Fatty Acids, Volatile/*metabolism
MH  - Female
MH  - Fermentation/*physiology
MH  - Firmicutes/*metabolism
MH  - Gastrointestinal Microbiome/*physiology
MH  - Humans
MH  - Lipogenesis/*physiology
MH  - Male
MH  - Pediatric Obesity/*metabolism/microbiology
PMC - PMC5095239
EDAT- 2016/11/05 06:00
MHDA- 2017/07/01 06:00
PMCR- 2017/11/01
CRDT- 2016/09/21 06:00
PHST- 2016/11/05 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
PHST- 2016/09/21 06:00 [entrez]
PHST- 2017/11/01 00:00 [pmc-release]
AID - 16-1797 [pii]
AID - 10.1210/jc.2016-1797 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2016 Nov;101(11):4367-4376. doi: 10.1210/jc.2016-1797. 
      Epub 2016 Sep 20.