PMID- 27650198
OWN - NLM
STAT- MEDLINE
DCOM- 20170921
LR  - 20180126
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 131
DP  - 2016 Dec
TI  - A simplified mycobacterial growth inhibition assay (MGIA) using direct infection 
      of mouse splenocytes and the MGIT system.
PG  - 7-9
LID - S0167-7012(16)30260-3 [pii]
LID - 10.1016/j.mimet.2016.09.010 [doi]
AB  - We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for 
      pre-clinical assessment of vaccine-mediated protection in mice. The assay is 
      accomplished by directly infecting splenocytes from vaccinated mice with 
      Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial 
      Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this 
      assay correlated with protection.
CI  - Published by Elsevier B.V.
FAU - Yang, Amy L
AU  - Yang AL
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA.
FAU - Schmidt, Thomas E
AU  - Schmidt TE
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA.
FAU - Stibitz, Scott
AU  - Stibitz S
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA.
FAU - Derrick, Steven C
AU  - Derrick SC
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA. Electronic address: 
      steven.derrick@fda.hhs.gov.
FAU - Morris, Sheldon L
AU  - Morris SL
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA.
FAU - Parra, Marcela
AU  - Parra M
AD  - Center for Biologics Evaluation and Research, United States Food and Drug 
      Administration, Silver Spring, MD, USA.
LA  - eng
GR  - FD/FDA HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20160917
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (BCG Vaccine)
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - BCG Vaccine/*immunology
MH  - Bacteriological Techniques/*methods
MH  - Cells, Cultured
MH  - Colony Count, Microbial
MH  - Communicable Diseases/*diagnosis
MH  - Culture Media
MH  - Disease Models, Animal
MH  - Drug Evaluation
MH  - Mice
MH  - Mycobacterium/*growth & development
MH  - Mycobacterium tuberculosis/growth & development
MH  - Spleen/immunology/*microbiology
MH  - Tuberculosis/immunology
OTO - NOTNLM
OT  - BCG
OT  - MGIA
OT  - MGIT
OT  - Tuberculosis
OT  - Vaccine
EDAT- 2016/10/26 06:00
MHDA- 2017/09/22 06:00
CRDT- 2016/11/07 06:00
PHST- 2016/08/29 00:00 [received]
PHST- 2016/09/15 00:00 [revised]
PHST- 2016/09/15 00:00 [accepted]
PHST- 2016/10/26 06:00 [pubmed]
PHST- 2017/09/22 06:00 [medline]
PHST- 2016/11/07 06:00 [entrez]
AID - S0167-7012(16)30260-3 [pii]
AID - 10.1016/j.mimet.2016.09.010 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2016 Dec;131:7-9. doi: 10.1016/j.mimet.2016.09.010. Epub 
      2016 Sep 17.