PMID- 27651910
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160921
LR  - 20220409
IS  - 2052-4897 (Print)
IS  - 2052-4897 (Electronic)
IS  - 2052-4897 (Linking)
VI  - 4
IP  - 1
DP  - 2016
TI  - Systemic inflammatory responses in patients with type 2 diabetes with chronic 
      periodontitis.
PG  - e000260
LID - 10.1136/bmjdrc-2016-000260 [doi]
LID - e000260
AB  - OBJECTIVE: The objective of this case-control study was to quantify the immune 
      responsiveness in individuals with type 2 diabetes (T2D) as compared with 
      patients without diabetes (NT2D) diagnosed with periodontitis. RESEARCH DESIGN 
      AND METHODS: Peripheral blood was collected from 20 patients with 
      moderate-to-severe chronic periodontitis (10 T2D, 10 NT2D). Blood samples were 
      stimulated with ultrapure Porphyromonas gingivalis and Escherichia coli 
      lipopolysaccharide (LPS) for 24 hours. 14 cytokines/chemokines were quantified in 
      culture supernatants using multiplex technology. RESULTS: T2D individuals 
      demonstrated higher unstimulated levels of interleukin 6 (IL-6), IL-1beta, tumor 
      necrosis factor alpha, interferon gamma, IL-10, IL-8, macrophage inflammatory protein 1alpha 
      (MIP1alpha), and 1beta (MIP1beta), and higher stimulated levels of IL-6, IL-8, IL-10, MIP1alpha 
      and MIP1beta, along with lower unstimulated and stimulated levels of 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) when compared with NT2D 
      (p<0.05). Importantly, the LPS-induced levels of IL-6, IL-8, IL-10 and MIP1alpha 
      strongly correlated with severity of disease, measured by pocket depths (PD), 
      within the T2D group (r(2)>/=0.7, p<0.05), but not within NT2D. CONCLUSIONS: Among 
      patients with chronic periodontitis, patients with T2D seem to have an enhanced 
      LPS-induced immune responsiveness than individuals without diabetes, which 
      correlates with periodontal disease severity, concomitant with a less robust 
      GM-CSF response. This data may in part explain the higher predisposition to 
      periodontitis in this population.
FAU - Mesia, Ruben
AU  - Mesia R
AD  - Department of Periodontology , University of Florida College of Dentistry , 
      Gainesville, Florida , USA.
FAU - Gholami, Fatemeh
AU  - Gholami F
AD  - Department of Periodontology , University of Florida College of Dentistry , 
      Gainesville, Florida , USA.
FAU - Huang, Hong
AU  - Huang H
AD  - Department of Periodontology , University of Florida College of Dentistry , 
      Gainesville, Florida , USA.
FAU - Clare-Salzler, Michael
AU  - Clare-Salzler M
AD  - Department of Endocrinology, Diabetes and Metabolism , University of Florida 
      College of Medicine , Gainesville, Florida , USA.
FAU - Aukhil, Ikramuddin
AU  - Aukhil I
AD  - Department of Periodontology , University of Florida College of Dentistry , 
      Gainesville, Florida , USA.
FAU - Wallet, Shannon M
AU  - Wallet SM
AD  - Department of Oral Biology , University of Florida College of Dentistry , 
      Gainesville, Florida , USA.
FAU - Shaddox, Luciana M
AU  - Shaddox LM
AD  - Department of Periodontology, University of Florida College of Dentistry, 
      Gainesville, Florida, USA; Department of Oral Biology, University of Florida 
      College of Dentistry, Gainesville, Florida, USA.
LA  - eng
PT  - Journal Article
DEP - 20160908
PL  - England
TA  - BMJ Open Diabetes Res Care
JT  - BMJ open diabetes research & care
JID - 101641391
PMC - PMC5020743
OTO - NOTNLM
OT  - Chronic Diabetic Complications
OT  - Chronic Inflammation
OT  - Cytokine(s)
OT  - Immunology
EDAT- 2016/09/22 06:00
MHDA- 2016/09/22 06:01
PMCR- 2016/09/08
CRDT- 2016/09/22 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/06/10 00:00 [revised]
PHST- 2016/07/05 00:00 [accepted]
PHST- 2016/09/22 06:00 [entrez]
PHST- 2016/09/22 06:00 [pubmed]
PHST- 2016/09/22 06:01 [medline]
PHST- 2016/09/08 00:00 [pmc-release]
AID - bmjdrc-2016-000260 [pii]
AID - 10.1136/bmjdrc-2016-000260 [doi]
PST - epublish
SO  - BMJ Open Diabetes Res Care. 2016 Sep 8;4(1):e000260. doi: 
      10.1136/bmjdrc-2016-000260. eCollection 2016.