PMID- 27652169
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160921
LR  - 20201001
IS  - 2193-1801 (Print)
IS  - 2193-1801 (Electronic)
IS  - 2193-1801 (Linking)
VI  - 5
IP  - 1
DP  - 2016
TI  - Microbial nitrification in throughfall of a Japanese cedar associated with 
      archaea from the tree canopy.
PG  - 1596
LID - 10.1186/s40064-016-3286-y [doi]
LID - 1596
AB  - To investigate the nitrification potential of phyllospheric microbes, we 
      incubated throughfall samples collected under the canopies of Japanese cedar 
      (Cryptomeria japonica) and analyzed the transformation of inorganic nitrogen in 
      the samples. Nitrate concentration increased in the unfiltered throughfall after 
      4 weeks of incubation, but remained nearly constant in the filtered samples (pore 
      size: 0.2 and 0.4 microm). In the unfiltered samples, delta(18)O and delta(15)N values of 
      nitrate decreased during incubation. In addition, archaeal ammonia monooxygenase 
      subunit A (amoA) genes, which participate in the oxidation of ammonia, were found 
      in the throughfall samples, although betaproteobacterial amoA genes were not 
      detected. The amoA genes recovered from the leaf surface of C. japonica were also 
      from archaea. Conversely, nitrate production, decreased isotope ratios of 
      nitrate, and the presence of amoA genes was not observed in rainfall samples 
      collected from an open area. Thus, the microbial nitrification that occurred in 
      the incubated throughfall is likely due to ammonia-oxidizing archaea that were 
      washed off the tree canopy by precipitation.
FAU - Watanabe, Keiji
AU  - Watanabe K
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan ; Center for Environmental Science in Saitama, Kazo, Saitama 
      347-0115 Japan.
FAU - Kohzu, Ayato
AU  - Kohzu A
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Suda, Wataru
AU  - Suda W
AD  - Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba 
      277-8562 Japan.
FAU - Yamamura, Shigeki
AU  - Yamamura S
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Takamatsu, Takejiro
AU  - Takamatsu T
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Takenaka, Akio
AU  - Takenaka A
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Koshikawa, Masami Kanao
AU  - Koshikawa MK
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Hayashi, Seiji
AU  - Hayashi S
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
FAU - Watanabe, Mirai
AU  - Watanabe M
AD  - National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 
      305-8506 Japan.
LA  - eng
PT  - Journal Article
DEP - 20160917
PL  - Switzerland
TA  - Springerplus
JT  - SpringerPlus
JID - 101597967
PMC - PMC5026986
OTO - NOTNLM
OT  - Ammonia-oxidizing archaea
OT  - Nitrification
OT  - Phyllosphere
OT  - Throughfall
OT  - amoA
EDAT- 2016/09/22 06:00
MHDA- 2016/09/22 06:01
PMCR- 2016/09/17
CRDT- 2016/09/22 06:00
PHST- 2015/07/06 00:00 [received]
PHST- 2016/09/11 00:00 [accepted]
PHST- 2016/09/22 06:00 [entrez]
PHST- 2016/09/22 06:00 [pubmed]
PHST- 2016/09/22 06:01 [medline]
PHST- 2016/09/17 00:00 [pmc-release]
AID - 3286 [pii]
AID - 10.1186/s40064-016-3286-y [doi]
PST - epublish
SO  - Springerplus. 2016 Sep 17;5(1):1596. doi: 10.1186/s40064-016-3286-y. eCollection 
      2016.