PMID- 27653181
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 47
IP  - 9
DP  - 2017 Aug
TI  - High-throughput sequencing to identify microRNA signatures during hepatic 
      differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem 
      cells.
PG  - 910-927
LID - 10.1111/hepr.12820 [doi]
AB  - AIM: MicroRNAs (miRNAs) constitute a class of small non-coding RNAs involved in 
      regulation of cognate mRNAs post-transcriptionally. MicroRNAs have been 
      implicated in regulating the stem cell differentiation process. Limited 
      regulatory miRNAs have been reported to date during hepatic differentiation of 
      stem cells. The present study was designed to identify the signature miRNAs 
      implicated in hepatic differentiation of stem cells using next-generation 
      sequencing methods. METHODS: We undertook sequencing of miRNAs isolated from 
      three different time points during hepatic differentiation of human umbilical 
      cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) from two 
      biological replicates. RESULTS: Out of a total known 2588 miRNAs (according to 
      miRBase version 21), 880 miRNAs were identified in our study. A total of 63 
      significantly expressed miRNAs during hepatic differentiation, with at least 
      2-fold change and a false discovery rate value <0.05, were considered for further 
      analysis. The putative target genes of significantly downregulated miRNAs during 
      hepatic differentiation appeared to be mostly associated with biological 
      processes that are essential for hepatic differentiation and maintenance of 
      mature hepatic phenotype-like liver development, stem cell differentiation, Wnt 
      receptor signaling pathway, and drug and cholesterol metabolic processes. 
      Putative target genes of significantly upregulated miRNAs are highly enriched in 
      regulating processes that block hepatic differentiation of hUC-MSCs like 
      epithelial-mesenchymal transition, transforming growth factor-beta receptor 
      signaling pathway, and stem cell maintenance. CONCLUSION: The study provides a 
      new insight for investigation of miRNA-regulated pathways during the 
      differentiation process.
CI  - (c) 2016 The Japan Society of Hepatology.
FAU - Raut, Akshata
AU  - Raut A
AD  - Department of Biological Sciences, Sunandan Divatia School of Science, Narsee 
      Monjee Institute of Management Studies University, Mumbai, India.
FAU - Khanna, Aparna
AU  - Khanna A
AD  - Department of Biological Sciences, Sunandan Divatia School of Science, Narsee 
      Monjee Institute of Management Studies University, Mumbai, India.
LA  - eng
PT  - Journal Article
DEP - 20161027
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
OTO - NOTNLM
OT  - hepatic differentiation
OT  - human umbilical cord
OT  - mesenchymal stem cells
OT  - miRNA expression profiling
OT  - microRNA
OT  - next-generation sequencing
EDAT- 2016/10/28 06:00
MHDA- 2016/10/28 06:01
CRDT- 2016/09/23 06:00
PHST- 2016/05/25 00:00 [received]
PHST- 2016/08/10 00:00 [revised]
PHST- 2016/09/17 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2016/10/28 06:01 [medline]
PHST- 2016/09/23 06:00 [entrez]
AID - 10.1111/hepr.12820 [doi]
PST - ppublish
SO  - Hepatol Res. 2017 Aug;47(9):910-927. doi: 10.1111/hepr.12820. Epub 2016 Oct 27.