PMID- 2765526
OWN - NLM
STAT- MEDLINE
DCOM- 19891006
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 28
IP  - 12
DP  - 1989 Jun 13
TI  - Dynamics of Antarctic fish microtubules at low temperatures.
PG  - 5089-95
AB  - The tubulins of Antarctic fishes, purified from brain tissue and depleted of 
      microtubule-associated proteins (MAPs), polymerized efficiently in vitro to yield 
      microtubules at near-physiological and supraphysiological temperatures (5, 10, 
      and 20 degrees C). The dynamics of the microtubules at these temperatures were 
      examined through the use of labeled guanosine 5'-triphosphate (GTP) as a marker 
      for the incorporation, retention, and loss of tubulin dimers. Following 
      attainment of a steady state in microtubule mass at 20 degrees C, the rate of 
      incorporation of [3H]GTP (i.e., tubulin dimers) during pulses of constant 
      duration decreased asymptotically toward a constant, nonzero value as the 
      interval prior to label addition to the microtubule solution increased. 
      Concomitant with the decreasing rate of label incorporation, the average length 
      of the microtubules increased, and the number concentration of microtubules 
      decreased. Thus, redistribution of microtubule lengths (probably via dynamic 
      instability and/or microtubule annealing) appears to be responsible for the 
      time-dependent decrease in the rate of tubulin uptake. When the microtubules had 
      attained both a steady state in mass and a constant length distribution, linear 
      incorporation of labeled tubulin dimers over time occurred at rates of 1.45 s-1 
      at 5 degrees C, 0.48 s-1 at 10 degrees C, and 0.18 s-1 at 20 degrees C. Thus, the 
      microtubules displayed greater rates of subunit flux, or treadmilling, at lower, 
      near-physiological temperatures. At each temperature, most of the incorporated 
      label was retained by the microtubules during a subsequent chase with excess 
      unlabeled GTP.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Himes, R H
AU  - Himes RH
AD  - Department of Biochemistry, University of Kansas, Lawrence 66045.
FAU - Detrich, H W 3rd
AU  - Detrich HW 3rd
LA  - eng
GR  - GM-36953/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Biopolymers)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Tubulin)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Animals
MH  - Biopolymers
MH  - *Brain Chemistry
MH  - Fishes
MH  - Guanosine Triphosphate/metabolism
MH  - Microscopy, Electron
MH  - Microtubule-Associated Proteins/isolation & purification
MH  - Microtubules/*metabolism/physiology/ultrastructure
MH  - Temperature
MH  - Tubulin/isolation & purification/*metabolism
EDAT- 1989/06/13 00:00
MHDA- 1989/06/13 00:01
CRDT- 1989/06/13 00:00
PHST- 1989/06/13 00:00 [pubmed]
PHST- 1989/06/13 00:01 [medline]
PHST- 1989/06/13 00:00 [entrez]
AID - 10.1021/bi00438a028 [doi]
PST - ppublish
SO  - Biochemistry. 1989 Jun 13;28(12):5089-95. doi: 10.1021/bi00438a028.