PMID- 27656642
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160922
LR  - 20220716
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 4
DP  - 2016
TI  - Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research 
      Applications.
PG  - 89
LID - 10.3389/fcell.2016.00089 [doi]
LID - 89
AB  - Fluorescence in situ hybridization (FISH) is a macromolecule recognition 
      technology based on the complementary nature of DNA or DNA/RNA double strands. 
      Selected DNA strands incorporated with fluorophore-coupled nucleotides can be 
      used as probes to hybridize onto the complementary sequences in tested cells and 
      tissues and then visualized through a fluorescence microscope or an imaging 
      system. This technology was initially developed as a physical mapping tool to 
      delineate genes within chromosomes. Its high analytical resolution to a single 
      gene level and high sensitivity and specificity enabled an immediate application 
      for genetic diagnosis of constitutional common aneuploidies, 
      microdeletion/microduplication syndromes, and subtelomeric rearrangements. FISH 
      tests using panels of gene-specific probes for somatic recurrent losses, gains, 
      and translocations have been routinely applied for hematologic and solid tumors 
      and are one of the fastest-growing areas in cancer diagnosis. FISH has also been 
      used to detect infectious microbias and parasites like malaria in human blood 
      cells. Recent advances in FISH technology involve various methods for improving 
      probe labeling efficiency and the use of super resolution imaging systems for 
      direct visualization of intra-nuclear chromosomal organization and profiling of 
      RNA transcription in single cells. Cas9-mediated FISH (CASFISH) allowed in situ 
      labeling of repetitive sequences and single-copy sequences without the disruption 
      of nuclear genomic organization in fixed or living cells. Using oligopaint-FISH 
      and super-resolution imaging enabled in situ visualization of chromosome 
      haplotypes from differentially specified single-nucleotide polymorphism loci. 
      Single molecule RNA FISH (smRNA-FISH) using combinatorial labeling or sequential 
      barcoding by multiple round of hybridization were applied to measure mRNA 
      expression of multiple genes within single cells. Research applications of these 
      single molecule single cells DNA and RNA FISH techniques have visualized 
      intra-nuclear genomic structure and sub-cellular transcriptional dynamics of many 
      genes and revealed their functions in various biological processes.
FAU - Cui, Chenghua
AU  - Cui C
AD  - Laboratory of Clinical Cytogenetics, Department of Genetics, Yale School of 
      MedicineNew Haven, CT, USA; Department of Pathology, Institute of Hematology and 
      Blood Diseases Hospital, Chinese Academy of Medical SciencesTianjin, China.
FAU - Shu, Wei
AU  - Shu W
AD  - Laboratory of Clinical Cytogenetics, Department of Genetics, Yale School of 
      MedicineNew Haven, CT, USA; Department of Cell Biology and Genetics, Guangxi 
      Medical UniversityNanning, China.
FAU - Li, Peining
AU  - Li P
AD  - Laboratory of Clinical Cytogenetics, Department of Genetics, Yale School of 
      Medicine New Haven, CT, USA.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20160905
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC5011256
OTO - NOTNLM
OT  - Cas-9 mediated FISH (CASFISH)
OT  - aneuploidy
OT  - fluorescence in situ hybridization (FISH)
OT  - genetic diagnosis
OT  - microdeletion/microduplication syndromes
OT  - oligopaint-FISH
OT  - pathogenic copy number variants (CNV)
OT  - single molecule RNA FISH (smRNA-FISH)
EDAT- 2016/09/23 06:00
MHDA- 2016/09/23 06:01
PMCR- 2016/01/01
CRDT- 2016/09/23 06:00
PHST- 2016/05/01 00:00 [received]
PHST- 2016/08/11 00:00 [accepted]
PHST- 2016/09/23 06:00 [entrez]
PHST- 2016/09/23 06:00 [pubmed]
PHST- 2016/09/23 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2016.00089 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2016 Sep 5;4:89. doi: 10.3389/fcell.2016.00089. eCollection 
      2016.