PMID- 27656684
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160922
LR  - 20201001
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 2
IP  - 9
DP  - 2016 Sep
TI  - Purification of the subcellular compartment in which exogenous antigens undergo 
      endoplasmic reticulum-associated degradation from dendritic cells.
PG  - e00151
LID - 10.1016/j.heliyon.2016.e00151 [doi]
LID - e00151
AB  - Dendritic cells (DCs) are capable of processing and presenting exogenous antigens 
      using MHC class I molecules. This pathway is called antigen cross-presentation 
      and plays an important role in the stimulation of naive CD8(+) T cells for 
      infectious and tumor immunity. Our previous studies in DC2.4 cells and bone 
      marrow-derived DCs revealed that exogenously added ovalbumin (OVA) is processed 
      through endoplasmic reticulum (ER)-associated degradation (ERAD) for 
      cross-presentation. In this study, we aimed to further confirm these results by 
      purification of the subcellular compartment in which exogenous antigens undergo 
      ERAD from homogenates of DC2.4 cells pretreated with biotinylated OVA (bOVA). 
      bOVA-containing vesicles were purified using streptavidin (SA)-magnetic beads 
      from cell homogenates and were found to contain ER chaperones and ERAD components 
      together with proteins for antigen presentation. In purified microsomes, bOVA was 
      retained in membranous fractions and degraded by the ubiquitin proteasome system 
      in presence reticulocyte lysates and ATP. These results strongly suggested that 
      DCs processed and degraded exogenous antigens through ERAD for cross-presentation 
      in this purified subcellular compartment.
FAU - Imai, Jun
AU  - Imai J
AD  - Laboratory of Physiological Chemistry, Faculty of Pharmacy, Tkasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma 370-0033, Japan.
FAU - Otani, Mayu
AU  - Otani M
AD  - Laboratory of Physiological Chemistry, Faculty of Pharmacy, Tkasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma 370-0033, Japan.
FAU - Sakai, Takahiro
AU  - Sakai T
AD  - Laboratory of Physiological Chemistry, Faculty of Pharmacy, Tkasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma 370-0033, Japan.
FAU - Hatta, Shinichi
AU  - Hatta S
AD  - Laboratory of Physiological Chemistry, Faculty of Pharmacy, Tkasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma 370-0033, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160908
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC5021789
OTO - NOTNLM
OT  - Cell biology
OT  - Immunology
EDAT- 2016/09/23 06:00
MHDA- 2016/09/23 06:01
PMCR- 2016/09/08
CRDT- 2016/09/23 06:00
PHST- 2016/07/18 00:00 [received]
PHST- 2016/08/26 00:00 [accepted]
PHST- 2016/09/23 06:00 [entrez]
PHST- 2016/09/23 06:00 [pubmed]
PHST- 2016/09/23 06:01 [medline]
PHST- 2016/09/08 00:00 [pmc-release]
AID - S2405-8440(16)30875-1 [pii]
AID - e00151 [pii]
AID - 10.1016/j.heliyon.2016.e00151 [doi]
PST - epublish
SO  - Heliyon. 2016 Sep 8;2(9):e00151. doi: 10.1016/j.heliyon.2016.e00151. eCollection 
      2016 Sep.