PMID- 27658394
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20190320
IS  - 1936-0541 (Electronic)
IS  - 1936-0533 (Linking)
VI  - 10
IP  - 6
DP  - 2016 Nov
TI  - Functionally aberrant dendritic cell subsets and expression of DC-SIGN 
      differentiate acute from chronic HBV infection.
PG  - 916-923
AB  - BACKGROUND: Dendritic cells (DCs) promote pathogen recognition, uptake and 
      presentation of antigen through DC-specific intercellular adhesion molecule 
      3-grabbing non-integrin (DC-SIGN) and toll-like receptors (TLRs). AIMS AND 
      OBJECTIVES: We aimed to study temporal changes in DCs, TLRs and DC-SIGN during 
      acute viral hepatitis B (AVHB) infection and compare them to chronic (CHB) and to 
      investigate the earliest time point of activated pathogen recognition receptors 
      in hepatitis B viral infection. METHODS: We measured the frequencies of 
      circulating myeloid (mDC) and plasmacytoid (pDC) dendritic cells and IFN-alpha 
      production along with the expression of DC-SIGN and Toll Like Receptors (TLR's) 
      in HBV patients at different time points. Also investigated in healthy 
      volunteers, the dynamic changes in TLRs expression after receiving hepatitis B 
      vaccine. RESULTS: On follow-up of AVHB patients, we found the mDC population was 
      significantly higher at week 4 and 6 (p < 0.02, 0.01), whereas the pDC population 
      was unchanged at week 6 compared with week 0. Whereas frequencies of mDCs and 
      pDCs were found to be elevated in AVHB and CHB patients than HC (p < 0.00 and 
      0.01, respectively) but was comparable among AVHB vs CHB. The DCs in CHB patients 
      were functionally impaired with significantly low IFN-alpha production and low DCSIGN 
      expression (p < 0.04 and 0.00, respectively). Even after stimulation by TLR 
      agonists, no change was found in IFN-alpha production in CHB patients. MyD88 and 
      IL-6, IFN-alpha mRNA levels were also found down-regulated. Interestingly, on 
      follow-up after HBV vaccine, TLRs expression was found high at day 3 after 
      vaccination. DISCUSSION: The initial events of immune activation might be 
      responsible for modulating immune response. These novel observations would pave 
      the way for the development of antiviral strategies for chronic HBV infection.
FAU - Sukriti, Sukriti
AU  - Sukriti S
AD  - Department of Molecular and Cellular Medicine, Institute of Liver and Biliary 
      Sciences, Delhi, India.
FAU - Trehanpati, Nirupma
AU  - Trehanpati N
AD  - Department of Molecular and Cellular Medicine, Institute of Liver and Biliary 
      Sciences, Delhi, India.
FAU - Kumar, Manoj
AU  - Kumar M
AD  - Department of Hepatology, Institute of Liver and Biliary Sciences, Delhi, India.
FAU - Pande, Chandana
AU  - Pande C
AD  - Department of Molecular and Cellular Medicine, Institute of Liver and Biliary 
      Sciences, Delhi, India.
FAU - Hissar, Syed S
AU  - Hissar SS
AD  - Department of Molecular and Cellular Medicine, Institute of Liver and Biliary 
      Sciences, Delhi, India.
FAU - Sarin, Shiv Kumar
AU  - Sarin SK
AD  - Department of Molecular and Cellular Medicine, Institute of Liver and Biliary 
      Sciences, Delhi, India. sksarin@ilbs.in.
AD  - Department of Hepatology, Institute of Liver and Biliary Sciences, Delhi, India. 
      sksarin@ilbs.in.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160922
PL  - United States
TA  - Hepatol Int
JT  - Hepatology international
JID - 101304009
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Hepatitis B Vaccines)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Adult
MH  - Cell Adhesion Molecules/*metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Hepatitis B/*immunology
MH  - Hepatitis B Vaccines/administration & dosage/immunology
MH  - Hepatitis B, Chronic/*immunology
MH  - Humans
MH  - Interferon-alpha/blood/genetics
MH  - Interleukin-6/genetics
MH  - Lectins, C-Type/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Myeloid Differentiation Factor 88/genetics
MH  - Receptors, Cell Surface/*metabolism
MH  - Toll-Like Receptors
MH  - Young Adult
OTO - NOTNLM
OT  - DC-SIGN
OT  - Dendritic cell subsets
OT  - Hepatitis B
OT  - Toll-like receptors
EDAT- 2016/10/28 06:00
MHDA- 2019/03/21 06:00
CRDT- 2016/09/24 06:00
PHST- 2015/10/21 00:00 [received]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2016/09/24 06:00 [entrez]
AID - 10.1007/s12072-016-9763-0 [pii]
AID - 10.1007/s12072-016-9763-0 [doi]
PST - ppublish
SO  - Hepatol Int. 2016 Nov;10(6):916-923. doi: 10.1007/s12072-016-9763-0. Epub 2016 
      Sep 22.