PMID- 27659198
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 16
IP  - 1
DP  - 2016 Sep 23
TI  - Human leukocyte antigen class 1 genotype distribution and analysis in persons 
      with active tuberculosis and household contacts from Central Uganda.
PG  - 504
LID - 504
AB  - BACKGROUND: To determine the distribution of Human leukocyte antigen (HLA) class 
      I genotypes in a Ugandan population of persons with tuberculosis (TB) and 
      establish the relationship between class I HLA types and Mycobacterium 
      tuberculosis (MTB) disease. METHODS: Blood samples were drawn from HIV negative 
      individuals with active TB and HIV negative household controls. DNA was extracted 
      from blood samples and HLA typed by the polymerase chain reaction-sequence 
      specific primer method. The allelic frequencies were determined by direct count. 
      RESULTS: HLA-A*02, B*15, C*07, C*03, B*58, C*04, A*01, A*74, C*02 and A*30 were 
      the dominant genotypes in this Ugandan cohort. There were differences in the 
      distribution of HLA types between the individuals with active TB and the 
      household controls with only HLA-A*03 allele showing a statistically significant 
      difference (p = 0.017 crude; OR = 6.29 and p = 0.016; OR = 11.67 after adjustment 
      for age). However, after applying the Benjamini and Hochberg adjustment for 
      multiple comparisons the difference was no longer statistically significant 
      (p = 0.374 and p = 0.176 respectively). CONCLUSIONS: We identified a number of 
      HLA class I alleles in a population from Central Uganda which will enable us to 
      carry out a functional characterization of CD8+ T-cell mediated immune responses 
      to MTB. Our results do not show a positive association between the HLA class I 
      alleles and TB in this Ugandan population however the study sample was too small 
      to draw any firm conclusions about the role of HLA class I alleles and TB 
      development in Uganda.
FAU - Buteme, Helen K
AU  - Buteme HK
AD  - Department of Medical Microbiology, School of Biomedical Sciences, College of 
      Health Sciences, Makerere University, P.O Box 7072, Kampala, Uganda. 
      hbuteme@gmail.com.
AD  - Department of Clinical Science and Education, Karolinska Institute, 
      Sodersjukhuset, SE-118 83, Stockholm, Sweden. hbuteme@gmail.com.
FAU - Axelsson-Robertson, Rebecca
AU  - Axelsson-Robertson R
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Nobels vag 16, KI Solna 
      Campus Karolinska Institute, Box 280, SE-171 77, Stockholm, Sweden.
FAU - Benson, Lina
AU  - Benson L
AD  - Department of Clinical Science and Education, Karolinska Institute, 
      Sodersjukhuset, SE-118 83, Stockholm, Sweden.
FAU - Joloba, Moses L
AU  - Joloba ML
AD  - Department of Medical Microbiology, School of Biomedical Sciences, College of 
      Health Sciences, Makerere University, P.O Box 7072, Kampala, Uganda.
FAU - Boom, W Henry
AU  - Boom WH
AD  - Tuberculosis Research Unit, Case Western Reserve University and University 
      Hospitals' Case Medical Center, 10900 Euclid Avenue, BRB 1031, Cleveland, OH, 
      44106-4984, USA.
FAU - Kallenius, Gunilla
AU  - Kallenius G
AD  - Department of Clinical Science and Education, Karolinska Institute, 
      Sodersjukhuset, SE-118 83, Stockholm, Sweden.
FAU - Maeurer, Markus
AU  - Maeurer M
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Nobels vag 16, KI Solna 
      Campus Karolinska Institute, Box 280, SE-171 77, Stockholm, Sweden.
LA  - eng
GR  - N01AI70022/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20160923
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
PMC - PMC5034515
OTO - NOTNLM
OT  - Genotype
OT  - HLA
OT  - Tuberculosis
OT  - Uganda
EDAT- 2016/09/24 06:00
MHDA- 2016/09/24 06:00
PMCR- 2016/09/23
CRDT- 2016/09/24 06:00
PHST- 2015/10/31 00:00 [received]
PHST- 2016/09/14 00:00 [accepted]
PHST- 2016/09/24 06:00 [entrez]
PHST- 2016/09/24 06:00 [pubmed]
PHST- 2016/09/24 06:00 [medline]
PHST- 2016/09/23 00:00 [pmc-release]
AID - 10.1186/s12879-016-1833-3 [pii]
AID - 1833 [pii]
AID - 10.1186/s12879-016-1833-3 [doi]
PST - epublish
SO  - BMC Infect Dis. 2016 Sep 23;16(1):504. doi: 10.1186/s12879-016-1833-3.