PMID- 27659399
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20180309
IS  - 1872-7484 (Electronic)
IS  - 1566-0702 (Linking)
VI  - 201
DP  - 2016 Dec
TI  - Adrenergic signaling elements in the bladder wall of the adult rat.
PG  - 40-48
LID - S1566-0702(16)30180-1 [pii]
LID - 10.1016/j.autneu.2016.09.002 [doi]
AB  - A growing body of work is describing the absence of a significant sympathetic 
      innervation of the detrusor implying little sympathetic regulation of bladder 
      contractility. However, low doses of adrenergic agonists are capable of relaxing 
      the bladder smooth muscle. If these effects underpin a physiological response 
      then the cellular nature and operation of this system are currently unknown. The 
      present immunohistochemistry study was done to explore the existence of 
      alternative adrenergic signaling elements in the rat bladder wall. Using 
      antibodies to tyrosine hydroxylase (TH) and vesicular mono-amine transporter 
      (vmat), few adrenergic nerves were found in the detrusor although TH 
      immunoreactive (IR) nerves were apparent in the bladder neck. TH-IR and vmat-IR 
      nerves were however abundant surrounding blood vessels. A population of vmat-IR 
      cells was found within the network of interstitial cells that surround the 
      detrusor muscle bundles. These vmat-IR cells were not or only weakly TH-IR. This 
      suggests that these interstitial cells have the capacity to store and release 
      catecholamines that may involve noradrenaline. Cells expressing the 
      beta(1)-adrenoceptor (beta(1)AR-IR) were also detected within the interstitial cell 
      network. Double staining with antibodies to beta(1)AR and vmat suggests that the 
      majority of vmat-IR interstitial cells show beta(1)AR-IR indicative of an autocrine 
      signaling system. In conclusion, a population of interstitial cells has the 
      machinery to store, release and respond to catecholamines. Thus, there might 
      exist a non-neuronal beta-adrenergic system operating in the bladder wall possibly 
      linked to one component of motor activity, micro-contractions, a system that may 
      be involved in mechanisms underpinning bladder sensation.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Persyn, Sara
AU  - Persyn S
AD  - Department of Urology, Antwerp University Hospital and University of Antwerp, 
      Faculty of Medicine, Antwerp, Belgium. Electronic address: 
      sarapersyn@hotmail.com.
FAU - Eastham, Jane
AU  - Eastham J
AD  - Uro-physiology Research Group, The Dental and Medical School, Newcastle 
      University, Newcastle upon Tyne NE2 4HH, England. Electronic address: 
      j.e.eastham@newcastle.ac.uk.
FAU - De Wachter, Stefan
AU  - De Wachter S
AD  - Department of Urology, Antwerp University Hospital and University of Antwerp, 
      Faculty of Medicine, Antwerp, Belgium. Electronic address: 
      stefan.dewachter@uantwerpen.be.
FAU - Gillespie, James
AU  - Gillespie J
AD  - Department of Urology, Antwerp University Hospital and University of Antwerp, 
      Faculty of Medicine, Antwerp, Belgium. Electronic address: 
      james.gillespie@uantwerpen.be.
LA  - eng
PT  - Journal Article
DEP - 20160910
PL  - Netherlands
TA  - Auton Neurosci
JT  - Autonomic neuroscience : basic & clinical
JID - 100909359
RN  - 0 (Catecholamines)
RN  - 0 (Receptors, Adrenergic, beta)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - Animals
MH  - Catecholamines/*metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Microscopy, Fluorescence
MH  - Rats, Wistar
MH  - Receptors, Adrenergic, beta/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Urinary Bladder/blood supply/cytology/innervation/*metabolism
MH  - Vesicular Monoamine Transport Proteins/metabolism
OTO - NOTNLM
OT  - Catecholamines
OT  - Interstitial cells
OT  - Rat
OT  - Urinary bladder
OT  - beta-Adrenoceptors
EDAT- 2016/09/24 06:00
MHDA- 2017/08/23 06:00
CRDT- 2016/09/24 06:00
PHST- 2016/05/08 00:00 [received]
PHST- 2016/09/04 00:00 [revised]
PHST- 2016/09/09 00:00 [accepted]
PHST- 2016/09/24 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
PHST- 2016/09/24 06:00 [entrez]
AID - S1566-0702(16)30180-1 [pii]
AID - 10.1016/j.autneu.2016.09.002 [doi]
PST - ppublish
SO  - Auton Neurosci. 2016 Dec;201:40-48. doi: 10.1016/j.autneu.2016.09.002. Epub 2016 
      Sep 10.