PMID- 27660010
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20170912
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 192
DP  - 2016 Nov 4
TI  - Therapeutic effect of Mahaenggamseok-tang on neutrophilic lung inflammation is 
      associated with NF-kappaB suppression and Nrf2 activation.
PG  - 486-495
LID - S0378-8741(16)30895-9 [pii]
LID - 10.1016/j.jep.2016.09.040 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Mahaenggamseok-tang (MHGST), an herbal formula in 
      traditional Asian medicine, has been used to treat patients with various 
      pulmonary diseases including common cold and influenza. However, the potential 
      therapeutic effect of MHGST on acute lung injury (ALI), a leading cause of death 
      worldwide, and the anti-inflammatory mechanisms of MHGST remained less 
      understood. MATERIALS AND METHODS: The methanol extract of MHGST was prepared and 
      fingerprinted by HPLC. For the induction of ALI, C57BL/6 mice (n=5/group) 
      received a single intraperitoneal (i.p.) injection of LPS. Referring to the dose 
      for patients, two different amounts of MHGST were delivered in an aerosol to 
      mouse lungs via trachea 2h after the i.p. LPS administration. Lung histology, 
      bronchoalveolar lavage fluid, myeloperoxidase (MPO) activity, and the expression 
      of inflammatory and Nrf2-dependent genes were analyzed to determine the effect of 
      MHGST on lung inflammation. For mechanistic studies, western blotting and 
      semi-quantitative RT-PCR were conducted using RAW 264.7 cells. RESULTS: When 
      administered 2h after the onset of ALI, MHGST relieved lung pathology 
      characteristic to ALI, with decreases of neutrophil infiltration and MPO 
      activity. While suppressing the expression of inflammatory genes, MHGST increased 
      the expression of Nrf2-dependent genes in ALI mouse lungs. Concordantly, MHGST 
      activated Nrf2 activity while suppressing NF-kappaB in RAW 264.7 cells. CONCLUSION: 
      MHGST suppressed neutrophilic lung inflammation, a hallmark of ALI, which was 
      associated with the activation of anti-inflammatory Nrf2 and the suppression of 
      pro-inflammatory NF-kappaB. Our results suggest that MHGST has a therapeutic 
      potential against ALI.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Kyun Ha
AU  - Kim KH
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea.
FAU - Lee, Ji Yeon
AU  - Lee JY
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea.
FAU - Kwun, Min Jung
AU  - Kwun MJ
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea.
FAU - Choi, Jun-Young
AU  - Choi JY
AD  - Department of Internal Medicine, Korean Medicine Hospital, Pusan National 
      University, Yangsan 50612, Republic of Korea.
FAU - Han, Chang Woo
AU  - Han CW
AD  - Department of Internal Medicine, Korean Medicine Hospital, Pusan National 
      University, Yangsan 50612, Republic of Korea.
FAU - Ha, Ki-Tae
AU  - Ha KT
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea.
FAU - Jeong, Seung-Il
AU  - Jeong SI
AD  - Jeonju Biomaterials Institute, Jeonju 561-360, Republic of Korea.
FAU - Jeong, Han-Sol
AU  - Jeong HS
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea. Electronic address: jhsol33@pusan.ac.kr.
FAU - Joo, Myungsoo
AU  - Joo M
AD  - School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of 
      Korea. Electronic address: mjoo@pusan.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160920
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (mahaenggamseok-tang)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Acute Lung Injury/chemically induced/metabolism/pathology/*prevention & control
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides
MH  - Lung/*drug effects/metabolism/pathology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-E2-Related Factor 2/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Neutrophil Infiltration/*drug effects
MH  - Peroxidase/metabolism
MH  - Pneumonia/chemically induced/metabolism/pathology/*prevention & control
MH  - RAW 264.7 Cells
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Acute lung injury
OT  - Anti-inflammation
OT  - Mahaenggamseok-tang
OT  - NF-kappaB
OT  - Nrf2
OT  - Therapeutic effect
EDAT- 2016/09/24 06:00
MHDA- 2017/05/16 06:00
CRDT- 2016/09/24 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/09/06 00:00 [revised]
PHST- 2016/09/18 00:00 [accepted]
PHST- 2016/09/24 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2016/09/24 06:00 [entrez]
AID - S0378-8741(16)30895-9 [pii]
AID - 10.1016/j.jep.2016.09.040 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2016 Nov 4;192:486-495. doi: 10.1016/j.jep.2016.09.040. Epub 
      2016 Sep 20.