PMID- 27661256
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 41
DP  - 2016 Oct 19
TI  - Film Thickness Determines Cell Growth and Cell Sheet Detachment from Spin-Coated 
      Poly(N-Isopropylacrylamide) Substrates.
PG  - 27564-27572
LID - 10.1021/acsami.6b09711 [doi]
AB  - Poly(N-isopropylacrylamide) (pNIPAm) is widely used to fabricate thermoresponsive 
      surfaces for cell sheet detachment. Many complex and expensive techniques have 
      been employed to produce pNIPAm substrates for cell culture. The spin-coating 
      technique allows rapid fabrication of pNIPAm substrates with high reproducibility 
      and uniformity. In this study, the dynamics of cell attachment, proliferation, 
      and function on non-cross-linked spin-coated pNIPAm films of different 
      thicknesses were investigated. The measurements of advancing contact angle 
      revealed increasing contact angles with increasing film thickness. Results 
      suggest that more hydrophilic 50 and 80 nm thin pNIPAm films are more preferable 
      for cell sheet fabrication, whereas more hydrophobic 300 and 900 nm thick 
      spin-coated pNIPAm films impede cell attachment. These changes in cell behavior 
      were correlated with changes in thickness and hydration of pNIPAm films. The 
      control of pNIPAm film thickness using the spin-coating technique offers an 
      effective tool for cell sheet-based tissue engineering.
FAU - Dzhoyashvili, Nina A
AU  - Dzhoyashvili NA
FAU - Thompson, Kerry
AU  - Thompson K
FAU - Gorelov, Alexander V
AU  - Gorelov AV
AD  - School of Chemistry and Chemical Biology, University College Dublin , D04 R7R0, 
      Belfield, Dublin 4, Ireland.
AD  - Institute of Theoretical and Experimental Biophysics, Russian Academy of Science 
      , 142290 Pushchino, Moscow Region, Russia.
FAU - Rochev, Yuri A
AU  - Rochev YA
AD  - Sechenov First Moscow State Medical University , Institute for Regenerative 
      Medicine, 119991 Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20161004
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
OTO - NOTNLM
OT  - cell sheet engineering
OT  - film thickness
OT  - pNIPAm
OT  - protein adsorption
OT  - spin-coating
OT  - wettability
EDAT- 2016/09/24 06:00
MHDA- 2016/09/24 06:01
CRDT- 2016/09/24 06:00
PHST- 2016/09/24 06:00 [pubmed]
PHST- 2016/09/24 06:01 [medline]
PHST- 2016/09/24 06:00 [entrez]
AID - 10.1021/acsami.6b09711 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Oct 19;8(41):27564-27572. doi: 
      10.1021/acsami.6b09711. Epub 2016 Oct 4.