PMID- 27661853
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220310
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 11
DP  - 2016 Sep 1
TI  - Fibrocytes and Fibrovascular Membrane Formation in Proliferative Diabetic 
      Retinopathy.
PG  - 4999-5005
LID - 10.1167/iovs.16-19798 [doi]
AB  - PURPOSE: The purpose of this study was to investigate whether fibrocytes 
      participate in formation of the fibrovascular membrane (FVM) in patients with 
      proliferative diabetic retinopathy (PDR). METHODS: Vitreous fluid and FVM samples 
      were obtained during vitrectomy in patients with PDR. Samples from patients with 
      macular hole or epiretinal membrane were used as controls. Vitreous fluid and FVM 
      samples were subjected to immunohistochemical analysis. In addition, cells 
      isolated from the vitreous fluid of PDR and control patients were cultured in 
      serum-free medium. Fibrocytes were identified among these cells by morphological 
      and immunohistochemical analyses. We examined the number of fibrocytes in PDR 
      patients and control patients. Also, the concentrations of monocyte 
      chemoattractant protein-1 (MCP-1), pentraxin3, and serum amyloid P (SAP) in 
      vitreous fluid samples from PDR patients and control patients were determined by 
      enzyme-linked immunosorbent assay. RESULTS: Fibrocytes were observed in the 
      vitreous and FVM samples from PDR patients. Cells cultured from the vitreous 
      samples of PDR patients were spindle shaped and expressed fibrocyte markers. 
      TGF-beta1 induced differentiation of these cells into myofibroblasts. The number of 
      fibrocytes was higher in samples from PDR patients than in samples from control 
      patient. The vitreous fluid concentration of MCP-1 was significantly higher in 
      PDR patients than in controls and showed a significant positive correlation with 
      the number of fibrocytes from the vitreous fluid. Vitreous fluid concentrations 
      of pentraxin3 and SAP were also higher in PDR patients than in control patients. 
      CONCLUSIONS: These findings indicate that fibrocytes may be involved in 
      development of the FVM in PDR.
FAU - Tamaki, Kazunori
AU  - Tamaki K
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, Chiba, Japan.
FAU - Usui-Ouchi, Ayumi
AU  - Usui-Ouchi A
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, Chiba, Japan.
FAU - Murakami, Akira
AU  - Murakami A
AD  - Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, 
      Japan.
FAU - Ebihara, Nobuyuki
AU  - Ebihara N
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, Chiba, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
EDAT- 2016/09/24 06:00
MHDA- 2016/09/24 06:01
CRDT- 2016/09/24 06:00
PHST- 2016/09/24 06:00 [entrez]
PHST- 2016/09/24 06:00 [pubmed]
PHST- 2016/09/24 06:01 [medline]
AID - 2556280 [pii]
AID - 10.1167/iovs.16-19798 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Sep 1;57(11):4999-5005. doi: 
      10.1167/iovs.16-19798.