PMID- 27663075
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20210816
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 439
DP  - 2017 Jan 5
TI  - CCAAT/enhancer binding protein beta negatively regulates progesterone receptor 
      expression in human glioblastoma cells.
PG  - 317-327
LID - S0303-7207(16)30383-5 [pii]
LID - 10.1016/j.mce.2016.09.018 [doi]
AB  - Many progesterone (P4) actions are mediated by its intracellular receptor (PR), 
      which has two isoforms (PR-A and PR-B) differentially transcribed from separate 
      promoters of a single gene. In glioblastomas, the most frequent and aggressive 
      brain tumors, PR-B is the predominant isoform. In an in silico analysis we showed 
      putative CCAAT/Enhancer Binding Protein (C/EBP) binding sites at PR-B promoter. 
      We evaluated the role of C/EBPbeta in PR-B expression regulation in glioblastoma 
      cell lines, which expressed different ratios of PR and C/EBPbeta isoforms (LAP1, 
      LAP2, and LIP). ChIP assays showed a significant basal binding of C/EBPbeta, 
      specific protein 1 (Sp1) and estrogen receptor alpha (ERalpha) to PR-B promoter. 
      C/EBPbeta knockdown increased PR-B expression and treatment with estradiol (E2) 
      reduced C/EBPbeta binding to the promoter and up-regulated PR-B expression. P4 
      induced genes were differently regulated when CEBP/beta was silenced. These data 
      show that C/EBPbeta negatively regulates PR-B expression in glioblastoma cells.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Hansberg-Pastor, Valeria
AU  - Hansberg-Pastor V
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico (UNAM), Mexico.
FAU - Gonzalez-Arenas, Aliesha
AU  - Gonzalez-Arenas A
AD  - Departamento de Medicina Genomica y Toxicologia Ambiental, Instituto de 
      Investigaciones Biomedicas, UNAM, Mexico.
FAU - Camacho-Arroyo, Ignacio
AU  - Camacho-Arroyo I
AD  - Unidad de Investigacion en Reproduccion Humana, Instituto Nacional de 
      Perinatologia-Facultad de Quimica, UNAM, Ciudad de Mexico, Mexico. Electronic 
      address: camachoarroyo@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160920
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Sp1 Transcription Factor)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Binding Sites/genetics
MH  - Brain Neoplasms/*genetics
MH  - CCAAT-Enhancer-Binding Protein-beta/*metabolism
MH  - Cell Line, Tumor
MH  - Chromatin Immunoprecipitation
MH  - Computer Simulation
MH  - Estradiol/pharmacology
MH  - Estrogen Receptor alpha/metabolism
MH  - *Gene Expression Regulation, Neoplastic/drug effects
MH  - Gene Knockdown Techniques
MH  - Gene Silencing/drug effects
MH  - Glioblastoma/*genetics
MH  - Humans
MH  - Progesterone/pharmacology
MH  - Promoter Regions, Genetic
MH  - Protein Binding/genetics
MH  - Protein Isoforms/genetics/metabolism
MH  - Receptors, Progesterone/*genetics/metabolism
MH  - Sp1 Transcription Factor/metabolism
OTO - NOTNLM
OT  - CCAAT-enhancer binding protein beta
OT  - Cancer
OT  - Gene expression
OT  - Glioblastoma
OT  - Progesterone receptor
OT  - Repressor
EDAT- 2016/09/25 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/09/25 06:00
PHST- 2016/05/30 00:00 [received]
PHST- 2016/08/29 00:00 [revised]
PHST- 2016/09/19 00:00 [accepted]
PHST- 2016/09/25 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/09/25 06:00 [entrez]
AID - S0303-7207(16)30383-5 [pii]
AID - 10.1016/j.mce.2016.09.018 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2017 Jan 5;439:317-327. doi: 10.1016/j.mce.2016.09.018. Epub 
      2016 Sep 20.