PMID- 27663077
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20181113
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 439
DP  - 2017 Jan 5
TI  - Gonadotropin releasing hormone activation of the mTORC2/Rictor complex regulates 
      actin remodeling and ERK activity in LbetaT2 cells.
PG  - 346-353
LID - S0303-7207(16)30386-0 [pii]
LID - 10.1016/j.mce.2016.09.021 [doi]
AB  - The mammalian target of rapamycin (mTOR) assembles into two different 
      multi-protein complexes, mTORC1 and mTORC2. The mTORC2 complex is distinct due to 
      the unique expression of the specific core regulatory protein Rictor 
      (rapamycin-insensitive companion of mTOR). mTORC2 has been implicated in 
      regulating actin cytoskeletal reorganization but its role in gonadotrope function 
      is unknown. Using the gonadotrope-derived LbetaT2 cell line, we find that the GnRH 
      agonist buserelin (GnRHa) phosphorylates both mTOR and Rictor. Interestingly, 
      inhibition of mTORC2 blunts GnRHa-induced cyto-architectural rearrangements. 
      Coincident with blunting of actin reorganization, inhibition of mTORC2 also 
      attenuates GnRHa-mediated activation of both protein kinase C (PKC) and 
      extracellular signal regulated kinase (ERK). Collectively, our data suggests that 
      GnRHa-mediated mTORC2 activation is important in facilitating actin 
      reorganization events critical for initiating PKC activity and subsequent ERK 
      phosphorylation in the gonadotrope-derived LbetaT2 cell line.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Edwards, Brian S
AU  - Edwards BS
AD  - Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, 
      USA. Electronic address: b80@uwyo.edu.
FAU - Isom, William J
AU  - Isom WJ
AD  - Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, 
      USA. Electronic address: wyoisom@gmail.com.
FAU - Navratil, Amy M
AU  - Navratil AM
AD  - Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, 
      USA. Electronic address: anavrati@uwyo.edu.
LA  - eng
GR  - P20 GM103432/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160920
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 2)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Actins/*metabolism
MH  - Carrier Proteins/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Gonadotrophs/drug effects/*metabolism
MH  - Gonadotropin-Releasing Hormone/metabolism/*pharmacology
MH  - Mechanistic Target of Rapamycin Complex 2/*metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Kinase C/metabolism
PMC - PMC5123956
MID - NIHMS819997
OTO - NOTNLM
OT  - Actin
OT  - ERK
OT  - Gonadotrope
OT  - Mammalian target of rapamycin complex 2
OT  - PKC
EDAT- 2016/09/25 06:00
MHDA- 2017/11/29 06:00
PMCR- 2018/01/05
CRDT- 2016/09/25 06:00
PHST- 2016/07/01 00:00 [received]
PHST- 2016/08/26 00:00 [revised]
PHST- 2016/09/19 00:00 [accepted]
PHST- 2016/09/25 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/09/25 06:00 [entrez]
PHST- 2018/01/05 00:00 [pmc-release]
AID - S0303-7207(16)30386-0 [pii]
AID - 10.1016/j.mce.2016.09.021 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2017 Jan 5;439:346-353. doi: 10.1016/j.mce.2016.09.021. Epub 
      2016 Sep 20.