PMID- 27664689 OWN - NLM STAT- MEDLINE DCOM- 20170123 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 217 DP - 2017 Feb 15 TI - The polyphenolics and carbohydrates as indicators of botanical and geographical origin of Serbian autochthonous clones of red spice paprika. PG - 705-715 LID - S0308-8146(16)31414-5 [pii] LID - 10.1016/j.foodchem.2016.09.038 [doi] AB - Spice peppers (Capsicum annuum L.) var. Lemeska and Lakosnicka paprika were investigated to evaluate their polyphenolic and carbohydrate profiles and antioxidant activity. A total of forty-nine polyphenolics were identified using ultrahigh-performance liquid chromatography (UHPLC) coupled to LTQ OrbiTrap mass analyzer. Twenty-five of them were quantified using available standards, while the other compounds were confirmed by exact mass search of their deprotonated molecule [M-H](-) and its MS(4) fragmentation. Thirteen carbohydrates were quantified using high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). Radical scavenging activity (RSA) ranged from 17.32 to 48.34mmol TE (Trolox equivalent)/kg DW (dry weight) and total phenolics content (TPC) was ranged between 7.03 and 14.92g GAE (gallic acid equivalents)/kg DW. To our best knowledge, five polyphenolic compounds were for the first time tentatively identified in paprika: 5-O-p-coumaroylquinic acid, luteolin 7-O-(2''-O-pentosyl-4''-O-hexosyl)hexoside, quercetin 3-O-(2''-O-hexosyl)rhamnoside, isorhamnetin 3-O-[6''-O-(5-hydroxyferuloyl)hexoside]-7-O-rhamnoside, and luteolin 7-O-[2''-O-(5'''-O-sinapoyl)pentosyl-6''-O-malonyl]hexoside. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Mudric, Sanja Z AU - Mudric SZ AD - Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia. FAU - Gasic, Uros M AU - Gasic UM AD - Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia. FAU - Dramicanin, Aleksandra M AU - Dramicanin AM AD - Innovation Center, Faculty of Chemistry Ltd, 11158 Belgrade, Serbia. FAU - Ciric, Ivanka Z AU - Ciric IZ AD - Innovation Center, Faculty of Chemistry Ltd, 11158 Belgrade, Serbia. FAU - Milojkovic-Opsenica, Dusanka M AU - Milojkovic-Opsenica DM AD - Chair of Analytical Chemistry and Centre of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia. FAU - Popovic-Dordevic, Jelena B AU - Popovic-Dordevic JB AD - Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia. FAU - Momirovic, Nebojsa M AU - Momirovic NM AD - Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia. FAU - Tesic, Zivoslav Lj AU - Tesic ZL AD - Chair of Analytical Chemistry and Centre of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia. Electronic address: ztesic@chem.bg.ac.rs. LA - eng PT - Journal Article DEP - 20160907 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Carbohydrates) RN - 0 (Plant Extracts) RN - 0 (Polyphenols) RN - 07X3IB4R4Z (3-methylquercetin) RN - 9IKM0I5T1E (Quercetin) RN - KUX1ZNC9J2 (Luteolin) SB - IM MH - Capsicum/*chemistry/classification MH - Carbohydrates/*analysis MH - Chromatography, High Pressure Liquid/methods MH - Geography MH - Luteolin/analysis MH - Plant Extracts/analysis MH - Polyphenols/*analysis MH - Quercetin/analogs & derivatives MH - Serbia MH - Spices/*analysis OTO - NOTNLM OT - Antioxidant activity OT - Autochthonous clones OT - Carbohydrates OT - LC-MS OT - Polyphenolics OT - Red spice paprika EDAT- 2016/09/25 06:00 MHDA- 2017/01/24 06:00 CRDT- 2016/09/25 06:00 PHST- 2016/06/09 00:00 [received] PHST- 2016/09/02 00:00 [revised] PHST- 2016/09/05 00:00 [accepted] PHST- 2016/09/25 06:00 [entrez] PHST- 2016/09/25 06:00 [pubmed] PHST- 2017/01/24 06:00 [medline] AID - S0308-8146(16)31414-5 [pii] AID - 10.1016/j.foodchem.2016.09.038 [doi] PST - ppublish SO - Food Chem. 2017 Feb 15;217:705-715. doi: 10.1016/j.foodchem.2016.09.038. Epub 2016 Sep 7.