PMID- 27664742
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20180512
IS  - 1618-0623 (Electronic)
IS  - 0944-5013 (Linking)
VI  - 192
DP  - 2016 Nov
TI  - Utilization of physiological and taxonomic fluorescent probes to study 
      Lactobacilli cells and response to pH challenge.
PG  - 239-246
LID - S0944-5013(16)30516-X [pii]
LID - 10.1016/j.micres.2016.07.011 [doi]
AB  - pH stress is recognized as an important feature for Lactobacillus in relation to 
      lifestyle and commercial utility. Hence, this study aims to investigate the cell 
      function of Lactobacilli cells subjected to pHs between 7.0 and 2.0. For this 
      purpose, the Lactobacilli isolates of vegetable origin were first hybridized with 
      fluorescent oligonucleotide rRNA probes for detecting Lactobacillus species. 
      Then, cells were exposed to pH stress and labelled with fluorescent probes, 
      carboxyfluorescein diacetate (CFDA) and propidium iodine (PI), which provided the 
      insight into esterase activity and membrane integrity of cells. Among isolates, 
      fluorescence in situ hybridization (FISH) enabled us to specifically detect L. 
      plantarum and L. brevis. Interestingly, FCM analysis revealed that at pHs between 
      7.0 and 4.0 the cell membrane was intact, while after the exposure at pH 3.0, and 
      2.0 became perturbed or impaired. Finally, L. brevis and L. plantarum differed 
      from each other in fluorescence labeling behaviour and culturability. However, 
      the results showed that the same standard protocol for labeling enables 
      discrimination of subpopulations of tested species. Depending on the species, the 
      substantial culturability loss was observed at pH 3.0 and 2.0. These results 
      suggest that the taxonomic and physiological fluorescent probes could be suitable 
      for in situ detection of specific bacteria and rapid assessment of the 
      physiological status of cells.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Olszewska, Magdalena A
AU  - Olszewska MA
AD  - Chair of Industrial and Food Microbiology, Faculty of Food Science, University of 
      Warmia and Mazury in Olsztyn, Plac Cieszynski 1, 10-726 Olsztyn, Poland. 
      Electronic address: magdalena.olszewska@uwm.edu.pl.
FAU - Kocot, Aleksandra M
AU  - Kocot AM
AD  - Chair of Industrial and Food Microbiology, Faculty of Food Science, University of 
      Warmia and Mazury in Olsztyn, Plac Cieszynski 1, 10-726 Olsztyn, Poland.
FAU - Nynca, Anna
AU  - Nynca A
AD  - Research and Education Center, Laboratory of Molecular Diagnostics, University of 
      Warmia and Mazury in Olsztyn, Prawochenskiego 5, 10-720 Olsztyn, Poland.
FAU - Laniewska-Trokenheim, Lucja
AU  - Laniewska-Trokenheim L
AD  - Chair of Industrial and Food Microbiology, Faculty of Food Science, University of 
      Warmia and Mazury in Olsztyn, Plac Cieszynski 1, 10-726 Olsztyn, Poland.
LA  - eng
PT  - Journal Article
DEP - 20160801
PL  - Germany
TA  - Microbiol Res
JT  - Microbiological research
JID - 9437794
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Bacterial Physiological Phenomena
MH  - Flow Cytometry
MH  - Fluorescent Dyes
MH  - *Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Lactobacillus/*classification/*physiology
MH  - Microbial Viability
MH  - RNA, Ribosomal, 16S/genetics
OTO - NOTNLM
OT  - Epifluorescence microscopy
OT  - Flow cytometry
OT  - Fluorescence in situ hybridization
OT  - Plate counts
OT  - Survival
OT  - pH
EDAT- 2016/09/25 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/09/25 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/05/29 00:00 [revised]
PHST- 2016/07/31 00:00 [accepted]
PHST- 2016/09/25 06:00 [entrez]
PHST- 2016/09/25 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - S0944-5013(16)30516-X [pii]
AID - 10.1016/j.micres.2016.07.011 [doi]
PST - ppublish
SO  - Microbiol Res. 2016 Nov;192:239-246. doi: 10.1016/j.micres.2016.07.011. Epub 2016 
      Aug 1.