PMID- 27666488
OWN - NLM
STAT- MEDLINE
DCOM- 20170310
LR  - 20170310
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 36
IP  - 5
DP  - 2016 Nov
TI  - MicroRNAs modulate the expression of the SOX18 transcript in lung squamous cell 
      carcinoma.
PG  - 2884-2892
LID - 10.3892/or.2016.5102 [doi]
AB  - Recent statistics show that lung cancer is the second most common malignant tumor 
      in the world (14% of all cancers in the USA), both in terms of morbidity and 
      mortality. The mortality of this type of tumor shows an increasing trend (28% for 
      men and 26% for women). Lung squamous cell carcinoma (LSCC) is the second‑largest 
      histological subtype of non‑small cell lung cancers (NSCLCs) after 
      adenocarcinoma. SRY‑related HMG‑box 18 (SOX18) protein is an important 
      transcription factor involved in the development of the cardiovascular system and 
      the lymphatic ducts. In addition, it was observed that SOX18 functions in wound 
      healing processes and the development of atherosclerosis. Likewise, an increased 
      level of this protein was found in melanomas and malignant pancreatic, stomach 
      and breast tumors. Furthermore, high expression of SOX18 in gastric cancer 
      stromal cells was found to be associated with a poor patient prognosis. In the 
      present study, we analyzed the expression of the SOX18 protein and the mRNA level 
      in postoperative samples of LSCC and non‑malignant lung tissues (NMLTs), and a 
      disparity in both levels was observed. Because of the fact that 
      microRNAs (miRNAs) play important roles in the initiation and progression of lung 
      cancer, the main aim of this study was to identify the miRNAs that interact with 
      the SOX18 transcript in NSCLC cases. SOX18 mRNA expression level was 
      significantly lower in the LSCC tissues than that noted in the NMLTs (p<0.01). 
      However, protein levels were higher in the LSCC cases compared to these levels in 
      the NMLTs (p<0.0001). We showed that miR‑7a and miR‑24‑3p were expressed more 
      highly in the NMLTs than levels in the LSCC samples, and that they could be 
      switched off in lung cancer tissue. Additionally, correlations between RQ‑values 
      of SOX18 in NMLTs and LSCC samples (r=0.43, p=0.019), and between miR‑7a and 
      miR24‑3p in NMLT cases (r=0.4, p=0.057) as well as in the LSCC samples (r=0.51, 
      p=0.012) were noted. In conclusion, miRNAs interact with the mRNA of the SOX18 
      gene, but the mechanism by which they could be inhibited in cancer cells requires 
      further examination.
FAU - Olbromski, Mateusz
AU  - Olbromski M
AD  - Department of Histology and Embryology, Wroclaw Medical University, 50‑368 
      Wroclaw, Poland.
FAU - Grzegrzolka, Jedrzej
AU  - Grzegrzolka J
AD  - Department of Histology and Embryology, Wroclaw Medical University, 50‑368 
      Wroclaw, Poland.
FAU - Jankowska-Konsur, Alina
AU  - Jankowska-Konsur A
AD  - Department of Dermatology, Venereology and Allergology, Wroclaw Medical 
      University, 50‑368 Wroclaw, Poland.
FAU - Witkiewicz, Wojciech
AU  - Witkiewicz W
AD  - Regional Specialist Hospital, Research and Development Centre, 51‑124 Wroclaw, 
      Poland.
FAU - Podhorska-Okolow, Marzena
AU  - Podhorska-Okolow M
AD  - Department of Histology and Embryology, Wroclaw Medical University, 50‑368 
      Wroclaw, Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AD  - Department of Histology and Embryology, Wroclaw Medical University, 50‑368 
      Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20160919
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (SOX18 protein, human)
RN  - 0 (SOXF Transcription Factors)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Prognosis
MH  - RNA, Messenger/biosynthesis
MH  - SOXF Transcription Factors/*biosynthesis/genetics
EDAT- 2016/10/26 06:00
MHDA- 2017/03/11 06:00
CRDT- 2016/09/27 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/08/11 00:00 [accepted]
PHST- 2016/10/26 06:00 [pubmed]
PHST- 2017/03/11 06:00 [medline]
PHST- 2016/09/27 06:00 [entrez]
AID - 10.3892/or.2016.5102 [doi]
PST - ppublish
SO  - Oncol Rep. 2016 Nov;36(5):2884-2892. doi: 10.3892/or.2016.5102. Epub 2016 Sep 19.