PMID- 27667109
OWN - NLM
STAT- MEDLINE
DCOM- 20180308
LR  - 20181023
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 96
IP  - 34
DP  - 2016 Sep 13
TI  - [Endoplasmic reticulum stress in INS-1-3 cell associated with the expression 
      changes of MODY gene pathway].
PG  - 2739-2743
LID - 10.3760/cma.j.issn.0376-2491.2016.34.011 [doi]
AB  - Objective: To profile the gene expression changes associated with endoplasmic 
      reticulum stress in INS-1-3 cells induced by thapsigargin (TG) and tunicamycin 
      (TM). Methods: Normal cultured INS-1-3 cells were used as a control. TG and TM 
      were used to induce endoplasmic reticulum stress in INS-1-3 cells. Digital gene 
      expression profiling technique was used to detect differentially expressed gene. 
      The changes of gene expression were detected by expression pattern clustering 
      analysis, gene ontology (GO) function and pathway enrichment analysis. Real time 
      polymerase chain reaction (RT-PCR) was used to verify the key changes of gene 
      expression. Results: Compared with the control group, there were 57 (45 
      up-regulated, 12 down-regulated) and 135 (99 up-regulated, 36 down-regulated) 
      differentially expressed genes in TG and TM group, respectively. GO function 
      enrichment analyses indicated that the main enrichment was in the endoplasmic 
      reticulum. In signaling pathway analysis, the identified pathways were related 
      with endoplasmic reticulum stress, antigen processing and presentation, protein 
      export, and most of all, the maturity onset diabetes of the young (MODY) pathway. 
      Conclusion: Under the condition of endoplasmic reticulum stress, the related 
      expression changes of transcriptional factors in MODY signaling pathway may be 
      related with the impaired function in islet beta cells.
FAU - Liu, Y T
AU  - Liu YT
AD  - Laboratory of Endocrinology, China-Japan Friendship Hospital, Beijing 100029, 
      China.
FAU - Li, S R
AU  - Li SR
FAU - Wang, Z
AU  - Wang Z
FAU - Xiao, J Z
AU  - Xiao JZ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Hepatocyte Nuclear Factors)
RN  - 11089-65-9 (Tunicamycin)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Endoplasmic Reticulum
MH  - *Endoplasmic Reticulum Stress
MH  - Hepatocyte Nuclear Factors/*genetics
MH  - Signal Transduction
MH  - Thapsigargin
MH  - Tunicamycin
MH  - Up-Regulation
EDAT- 2016/09/27 06:00
MHDA- 2018/03/09 06:00
CRDT- 2016/09/27 06:00
PHST- 2016/09/27 06:00 [entrez]
PHST- 2016/09/27 06:00 [pubmed]
PHST- 2018/03/09 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2016.34.011 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2016 Sep 13;96(34):2739-2743. doi: 
      10.3760/cma.j.issn.0376-2491.2016.34.011.