PMID- 27668805
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 32
IP  - 41
DP  - 2016 Oct 18
TI  - Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and 
      Sensing.
PG  - 10776-10783
LID - 10.1021/acs.langmuir.6b03032 [doi]
AB  - Fluorescently labeled DNA adsorbed on graphene oxide (GO) is a well-established 
      sensing platform for detecting a diverse range of analytes. GO is a loosely 
      defined material and its oxygen content may vary depending on the condition of 
      preparation. Sometimes, a further reduction step is intentionally performed to 
      decrease the oxygen content, and the resulting material is called reduced GO 
      (rGO). In this study, DNA adsorption and desorption from GO and rGO is 
      systematically compared. Under the same salt concentration, DNA adsorbs slightly 
      faster with a 2.6-fold higher capacity on rGO. At the same time, DNA adsorbed on 
      rGO is more resistant to desorption induced by temperature, pH, urea, and organic 
      solvents. Various lengths and sequences of DNA probes have been tested. When its 
      complementary DNA is added as a model target analyte, the rGO sample has a higher 
      signal-to-background and signal-to-noise ratio, whereas the GO sample has a 
      slightly higher absolute signal increase and faster signaling kinetics. DNAs 
      adsorbed on GO or rGO are still susceptible to nonspecific displacement by other 
      DNA and proteins. Overall, although rGO adsorbs DNA more tightly, it allows 
      efficient DNA sensing with an extremely low background fluorescence signal.
FAU - Lu, Chang
AU  - Lu C
AD  - College of Biosystems Engineering and Food Science, Zhejiang University , 
      Hangzhou 310058, China.
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo , Waterloo N2L 3G1, Ontario, Canada.
FAU - Huang, Po-Jung Jimmy
AU  - Huang PJ
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo , Waterloo N2L 3G1, Ontario, Canada.
FAU - Liu, Biwu
AU  - Liu B
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo , Waterloo N2L 3G1, Ontario, Canada.
FAU - Ying, Yibin
AU  - Ying Y
AD  - College of Biosystems Engineering and Food Science, Zhejiang University , 
      Hangzhou 310058, China.
FAU - Liu, Juewen
AU  - Liu J
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo , Waterloo N2L 3G1, Ontario, Canada.
LA  - eng
PT  - Journal Article
DEP - 20161006
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
EDAT- 2016/09/27 06:00
MHDA- 2016/09/27 06:01
CRDT- 2016/09/27 06:00
PHST- 2016/09/27 06:00 [pubmed]
PHST- 2016/09/27 06:01 [medline]
PHST- 2016/09/27 06:00 [entrez]
AID - 10.1021/acs.langmuir.6b03032 [doi]
PST - ppublish
SO  - Langmuir. 2016 Oct 18;32(41):10776-10783. doi: 10.1021/acs.langmuir.6b03032. Epub 
      2016 Oct 6.