PMID- 27670158
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Sep 27
TI  - CD16 is indispensable for antibody-dependent cellular cytotoxicity by human 
      monocytes.
PG  - 34310
LID - 10.1038/srep34310 [doi]
LID - 34310
AB  - Antibody-dependent cellular cytotoxicity (ADCC) is exerted by immune cells 
      expressing surface Fcgamma receptors (FcgammaRs) against cells coated with antibody, such 
      as virus-infected or transformed cells. CD16, the FcgammaRIIIA, is essential for ADCC 
      by NK cells, and is also expressed by a subset of human blood monocytes. We found 
      that human CD16- expressing monocytes have a broad spectrum of ADCC capacities 
      and can kill cancer cell lines, primary leukemic cells and hepatitis B 
      virus-infected cells in the presence of specific antibodies. Engagement of CD16 
      on monocytes by antibody bound to target cells activated beta2-integrins and induced 
      TNFalpha secretion. In turn, this induced TNFR expression on the target cells, making 
      them susceptible to TNFalpha-mediated cell death. Treatment with TLR agonists, DAMPs 
      or cytokines, such as IFNgamma, further enhanced ADCC. Monocytes lacking CD16 did not 
      exert ADCC but acquired this property after CD16 expression was induced by either 
      cytokine stimulation or transient transfection. Notably, CD16+ monocytes from 
      patients with leukemia also exerted potent ADCC. Hence, CD16+ monocytes are 
      important effectors of ADCC, suggesting further developments of this property in 
      the context of cellular therapies for cancer and infectious diseases.
FAU - Yeap, Wei Hseun
AU  - Yeap WH
AD  - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research 
      (ASTAR), 8A Biomedical Grove, #04-06, Immunos, Singapore 138648, Singapore.
FAU - Wong, Kok Loon
AU  - Wong KL
AD  - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research 
      (ASTAR), 8A Biomedical Grove, #04-06, Immunos, Singapore 138648, Singapore.
FAU - Shimasaki, Noriko
AU  - Shimasaki N
AD  - Department of Pediatrics, National University of Singapore, Centre for 
      Translational Medicine, 14 Medical Drive Singapore 117599, Singapore.
FAU - Teo, Esmeralda Chi Yuan
AU  - Teo EC
AD  - Department of Haematology, Singapore General Hospital, Outram Road, Singapore 
      169608, Singapore.
FAU - Quek, Jeffrey Kim Siang
AU  - Quek JK
AD  - Department of Haematology, Singapore General Hospital, Outram Road, Singapore 
      169608, Singapore.
FAU - Yong, Hao Xiang
AU  - Yong HX
AD  - Department of Haematology, Singapore General Hospital, Outram Road, Singapore 
      169608, Singapore.
FAU - Diong, Colin Phipps
AU  - Diong CP
AD  - Department of Haematology, Singapore General Hospital, Outram Road, Singapore 
      169608, Singapore.
FAU - Bertoletti, Antonio
AU  - Bertoletti A
AD  - Singapore Institute for Clinical Sciences, Brenner Centre for Molecular Medicine, 
      30 Medical Drive, Singapore 117609, Singapore.
AD  - Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore.
FAU - Linn, Yeh Ching
AU  - Linn YC
AD  - Department of Haematology, Singapore General Hospital, Outram Road, Singapore 
      169608, Singapore.
FAU - Wong, Siew Cheng
AU  - Wong SC
AD  - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research 
      (ASTAR), 8A Biomedical Grove, #04-06, Immunos, Singapore 138648, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20160927
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
EIN - Sci Rep. 2017 Apr 07;7:46202. PMID: 28387358
PMC - PMC5037471
EDAT- 2016/09/28 06:00
MHDA- 2016/09/28 06:01
PMCR- 2016/09/27
CRDT- 2016/09/28 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/09/12 00:00 [accepted]
PHST- 2016/09/28 06:00 [entrez]
PHST- 2016/09/28 06:00 [pubmed]
PHST- 2016/09/28 06:01 [medline]
PHST- 2016/09/27 00:00 [pmc-release]
AID - srep34310 [pii]
AID - 10.1038/srep34310 [doi]
PST - epublish
SO  - Sci Rep. 2016 Sep 27;6:34310. doi: 10.1038/srep34310.