PMID- 27671067
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20220321
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 60
IP  - 12
DP  - 2016 Dec
TI  - Presence of the optrA Gene in Methicillin-Resistant Staphylococcus sciuri of 
      Porcine Origin.
PG  - 7200-7205
AB  - A total of 57 methicillin-resistant Staphylococcus aureus (MRSA) isolates and 475 
      methicillin-resistant coagulase-negative staphylococci (MRCoNS) collected from 
      pigs in the Guangdong province of China in 2014 were investigated for the 
      presence of the novel oxazolidinone-phenicol resistance gene optrA The optrA gene 
      was detected in 6.9% (n = 33) of the MRCoNS, all of which were Staphylococcus 
      sciuri isolates, but in none of the MRSA isolates. Five optrA-carrying 
      methicillin-resistant (MR) S. sciuri isolates also harbored the multiresistance 
      gene cfr Pulsed-field gel electrophoresis (PFGE) and dru typing of the 33 
      optrA-carrying MR S. sciuri isolates revealed 25 patterns and 5 sequence types, 
      respectively. S1 nuclease PFGE and Southern blotting confirmed that optrA was 
      located in the chromosomal DNAs of 29 isolates, including 1 cfr-positive isolate. 
      The remaining four isolates harbored a  approximately 35-kb pWo28-3-like plasmid on which optrA 
      and cfr were located together with other resistance genes, as confirmed by 
      sequence analysis. Six different types of genetic environments (types I to VI) of 
      the chromosome-borne optrA genes were identified; these types had the optrA gene 
      and its transcriptional regulator araC in common. Tn558 was found to be 
      associated with araC-optrA in types II to VI. The optrA gene in types II and III 
      was found in close proximity to the ccr gene complex of the respective 
      staphylococcal cassette chromosome mec element (SCCmec). Since oxazolidinones are 
      last-resort antimicrobial agents for the control of serious infections caused by 
      methicillin-resistant staphylococci in humans, the location of the optrA gene 
      close to the ccr complex is an alarming observation.
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Fan, Run
AU  - Fan R
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University, Beijing, People's Republic 
      of China.
FAU - Li, Dexi
AU  - Li D
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University, Beijing, People's Republic 
      of China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University, Beijing, People's Republic 
      of China.
FAU - He, Tao
AU  - He T
AD  - Institute of Food Safety and Detection, Jiangsu Academy of Agricultural Sciences, 
      Nanjing, People's Republic of China.
FAU - Fessler, Andrea T
AU  - Fessler AT
AD  - Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), 
      Neustadt-Mariensee, Germany.
AD  - Centre for Infection Medicine, Institute of Microbiology and Epizootics, 
      Department of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany.
FAU - Schwarz, Stefan
AU  - Schwarz S
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University, Beijing, People's Republic 
      of China wucm@cau.edu.cn stefan.schwarz@fli.bund.de.
AD  - Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), 
      Neustadt-Mariensee, Germany.
AD  - Centre for Infection Medicine, Institute of Microbiology and Epizootics, 
      Department of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany.
FAU - Wu, Congming
AU  - Wu C
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University, Beijing, People's Republic 
      of China wucm@cau.edu.cn stefan.schwarz@fli.bund.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161121
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (AraC Transcription Factor)
RN  - 0 (Bacterial Proteins)
RN  - 0 (CFR protein, Staphylococcus sciuri)
RN  - 0 (Oxazolidinones)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - AraC Transcription Factor/genetics
MH  - Bacterial Proteins/*genetics
MH  - China
MH  - Drug Resistance, Microbial/genetics
MH  - Drug Resistance, Multiple, Bacterial/*genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Methicillin Resistance/genetics
MH  - Molecular Typing
MH  - Oxazolidinones/*pharmacology
MH  - Staphylococcus/*drug effects/*genetics/isolation & purification
MH  - Swine
PMC - PMC5118999
EDAT- 2016/09/28 06:00
MHDA- 2017/10/03 06:00
PMCR- 2017/05/21
CRDT- 2016/09/28 06:00
PHST- 2016/07/23 00:00 [received]
PHST- 2016/09/06 00:00 [accepted]
PHST- 2016/09/28 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2016/09/28 06:00 [entrez]
PHST- 2017/05/21 00:00 [pmc-release]
AID - AAC.01591-16 [pii]
AID - 01591-16 [pii]
AID - 10.1128/AAC.01591-16 [doi]
PST - epublish
SO  - Antimicrob Agents Chemother. 2016 Nov 21;60(12):7200-7205. doi: 
      10.1128/AAC.01591-16. Print 2016 Dec.