PMID- 27680586
OWN - NLM
STAT- MEDLINE
DCOM- 20170717
LR  - 20201209
IS  - 1464-3405 (Electronic)
IS  - 0960-894X (Linking)
VI  - 26
IP  - 21
DP  - 2016 Nov 1
TI  - RNA fluorescence in situ hybridization using 3-cyanovinylcarbazole modified 
      oligodeoxyribonucleotides as photo-cross-linkable probes.
PG  - 5312-5314
LID - S0960-894X(16)30974-X [pii]
LID - 10.1016/j.bmcl.2016.09.035 [doi]
AB  - Photo-cross-linkable fluorescent oligodeoxyribonucleotides having 
      3-cyanovinylcarbazole nucleoside were applied to fluorescence in situ 
      hybridization (FISH) based 16S rRNA detection in Escherichia coli cells. As the 
      photo-cross-linked probe/rRNA hybrid was stable under the denaturing condition, 
      decrease of the fluorescence signal through the washing process was prevented. 
      The thermally irreversible hybridization property also enabled stable 
      hybridization with the structured region on the target RNA, and facilitated 
      design of the sequence for the FISH probe. Further development of the method 
      might contribute to quantitative and stable FISH staining.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Fujimoto, Kenzo
AU  - Fujimoto K
AD  - School of Materials Science, Japan Advanced Institute of Science and Technology, 
      1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan.
FAU - Toyosato, Kei
AU  - Toyosato K
AD  - School of Materials Science, Japan Advanced Institute of Science and Technology, 
      1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan.
FAU - Nakamura, Shigetaka
AU  - Nakamura S
AD  - School of Materials Science, Japan Advanced Institute of Science and Technology, 
      1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan.
FAU - Sakamoto, Takashi
AU  - Sakamoto T
AD  - School of Materials Science, Japan Advanced Institute of Science and Technology, 
      1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160915
PL  - England
TA  - Bioorg Med Chem Lett
JT  - Bioorganic & medicinal chemistry letters
JID - 9107377
RN  - 0 (Carbazoles)
RN  - 0 (Molecular Probes)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Carbazoles/*chemistry
MH  - Escherichia coli/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Probes
MH  - Oligodeoxyribonucleotides/*chemistry
MH  - *Photochemical Processes
MH  - RNA/*chemistry
OTO - NOTNLM
OT  - 16S rRNA
OT  - 3-Cyanovinylcarbazole nucleoside
OT  - Fluorescence in situ hybridization
OT  - Photo-cross-link
EDAT- 2016/10/26 06:00
MHDA- 2017/07/18 06:00
CRDT- 2016/09/30 06:00
PHST- 2016/07/26 00:00 [received]
PHST- 2016/08/29 00:00 [revised]
PHST- 2016/09/14 00:00 [accepted]
PHST- 2016/10/26 06:00 [pubmed]
PHST- 2017/07/18 06:00 [medline]
PHST- 2016/09/30 06:00 [entrez]
AID - S0960-894X(16)30974-X [pii]
AID - 10.1016/j.bmcl.2016.09.035 [doi]
PST - ppublish
SO  - Bioorg Med Chem Lett. 2016 Nov 1;26(21):5312-5314. doi: 
      10.1016/j.bmcl.2016.09.035. Epub 2016 Sep 15.