PMID- 27686451
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20181113
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 422
IP  - 1-2
DP  - 2016 Nov
TI  - Dihydromyricetin suppresses TNF-alpha-induced NF-kappaB activation and target gene 
      expression.
PG  - 11-20
AB  - Nuclear factor-kappa B (NF-kappaB) has been reported to play a pivotal role in many 
      physiological processes including inflammation, apoptosis, and angiogenesis. We 
      discovered a potent natural NF-kappaB inhibitor, dihydromyricetin, from the 
      traditional herb Ampelopsis grossedentata, which has a long history of use in 
      food and medicine. In this study, we demonstrated the effect of dihydromyricetin 
      on NF-kappaB activation in TNF-alpha-induced HeLa cells. Dihydromyricetin was found to 
      markedly inhibit the phosphorylation and degradation of the inhibitor of NF-kappaB 
      alpha (IkappaBalpha), and subsequent nuclear translocation of p65. Dihydromyricetin also 
      has an impact on upstream signaling of IKK through the inhibition of expression 
      of adaptor proteins, TNF receptor-associated factor 2 (TRAF2), and 
      receptor-interacting protein 1 (RIP1). Furthermore, the current results reveal 
      that dihydromyricetin led to the downregulation of target genes involved in 
      inflammation, proliferation, as well as potentiation of TNF-alpha-induced apoptosis 
      through suppressing the activation of NF-kappaB. In conclusion, our data indicate 
      that dihydromyricetin may be a potentially useful therapeutic agent for 
      inflammatory diseases.
FAU - Tang, Nina
AU  - Tang N
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Ma, Juan
AU  - Ma J
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Wang, Ke Si
AU  - Wang KS
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Mi, Chunliu
AU  - Mi C
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Lv, Ying
AU  - Lv Y
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Piao, Lian Xun
AU  - Piao LX
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Xu, Guang Hua
AU  - Xu GH
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Li, Xuezheng
AU  - Li X
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Lee, Jung Joon
AU  - Lee JJ
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China.
FAU - Jin, Xuejun
AU  - Jin X
AD  - Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, 
      Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, 
      Yanbian University, Yanji, 133002, Jilin Province, China. xjjin@ybu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160929
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (AGFG1 protein, human)
RN  - 0 (Flavonols)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (RELA protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - KD8QND6427 (dihydromyricetin)
SB  - IM
MH  - Active Transport, Cell Nucleus/drug effects
MH  - Apoptosis/*drug effects
MH  - Cell Nucleus/*metabolism
MH  - Flavonols/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - HeLa Cells
MH  - Humans
MH  - I-kappa B Kinase/metabolism
MH  - Nuclear Pore Complex Proteins/metabolism
MH  - RNA-Binding Proteins/metabolism
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Transcription Factor RelA/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - Apoptosis
OT  - Dihydromyricetin
OT  - Inflammation
OT  - IkappaBalpha
OT  - NF-kappaB
EDAT- 2016/10/01 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/10/01 06:00
PHST- 2016/03/16 00:00 [received]
PHST- 2016/08/17 00:00 [accepted]
PHST- 2016/10/01 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
PHST- 2016/10/01 06:00 [entrez]
AID - 10.1007/s11010-016-2799-6 [pii]
AID - 10.1007/s11010-016-2799-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2016 Nov;422(1-2):11-20. doi: 10.1007/s11010-016-2799-6. Epub 
      2016 Sep 29.