PMID- 27686854
OWN - NLM
STAT- MEDLINE
DCOM- 20170815
LR  - 20181202
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 32
IP  - 6
DP  - 2017 Jun
TI  - Ghrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration 
      in a rat model of epsilon-caprolactone conduit reconstruction.
PG  - 627-637
LID - 10.14670/HH-11-828 [doi]
AB  - OBJECTIVE: Attempts have been made to improve nerve conduits in peripheral nerve 
      reconstruction. We investigated the potential therapeutic effect of 
      adipose-derived mesenchymal cells (ASCs) and ghrelin (GHR), a neuropeptide with 
      neuroprotective, trophic, and developmental regulatory actions, on peripheral 
      nerve regeneration in a model of severe nerve injury repaired with nerve 
      conduits. MATERIAL AND METHODS: The right sciatic nerves of 24 male Wistar rats 
      were 10-mm transected unilaterally and repaired with Dl-lactic-epsilon-caprolactone 
      conduits. Rats were then treated locally with saline, ASCs, or GHR. At 12 weeks 
      post-surgery, we assessed limb function by measuring ankle stance angle and 
      percentage muscle mass reduction and evaluated the histopathology, 
      immunohistochemistry, ultrastructure, and morphometry of myelinated fibers. MAIN 
      RESULTS: Rats receiving GHR or ASCs showed no significant increased functional 
      recovery in ankle stance angle (p=0.372) but a higher nerve area (p=0.015), 
      myelin area (p=0.046) and number of myelinated fibers (p=0.012) in the middle and 
      distal segments of operated sciatic nerves in comparison to saline-treated 
      control animals. CONCLUSION: These results suggest that utilization of ghrelin or 
      ASCs may improve nerve regeneration using Dl-lactic-epsilon-caprolactone conduits.
FAU - Hernandez-Cortes, Pedro
AU  - Hernandez-Cortes P
AD  - Hand Surgery Unit, Orthopedic Surgery Department of San Cecilio University 
      Hospital of Granada, and Department of Surgery, University of Granada, Granada, 
      Spain.
FAU - Toledo-Romero, Miguel Angel
AU  - Toledo-Romero MA
AD  - Hand Surgery Unit, Orthopedic Surgery Department of Virgen del Rocio University 
      Hospital of Seville, Sevilla, Spain.
FAU - Delgado, Mario
AU  - Delgado M
AD  - Institute of Parasitology and Biomedicine Lopez-Neyra, CSIC, Granada, Spain.
FAU - Gonzalez-Rey, Elena
AU  - Gonzalez-Rey E
AD  - Institute of Parasitology and Biomedicine Lopez-Neyra, CSIC, Granada, Spain.
FAU - Gomez Sanchez, Rafael
AU  - Gomez Sanchez R
AD  - Hand Surgery Unit, Orthopedic Surgery Department of San Cecilio University 
      Hospital of Granada, and Department of Surgery, University of Granada, Granada, 
      Spain.
FAU - Prados-Olleta, Nicolas
AU  - Prados-Olleta N
AD  - Orthopedic Surgery Department of Virgen de las Nieves University Hospital of 
      Granada, and Department of Surgery, University of Granada, Spain.
FAU - Aneiros-Fernandez, Jose
AU  - Aneiros-Fernandez J
AD  - Department of Pathology, School of Medicine, and Biopathology and Regenerative 
      Medicine Institute (IBIMER), University of Granada, Granada, Spain.
FAU - Crespo-Lora, Vicente
AU  - Crespo-Lora V
AD  - Department of Pathology, School of Medicine, and Biopathology and Regenerative 
      Medicine Institute (IBIMER), University of Granada, Granada, Spain.
FAU - Aguilar, Mariano
AU  - Aguilar M
AD  - Department of Pathology, School of Medicine, and Biopathology and Regenerative 
      Medicine Institute (IBIMER), University of Granada, Granaada, Spain.
FAU - Galindo-Moreno, Pablo
AU  - Galindo-Moreno P
AD  - Oral Surgery and Implant Dentistry Department, School of Dentistry, University of 
      Granada, Granada, Spain.
FAU - O'Valle, Francisco
AU  - O'Valle F
AD  - Department of Pathology, School of Medicine, and Biopathology and Regenerative 
      Medicine Institute (IBIMER), University of Granada, Granada, Spain. 
      fovalle@ugr.es.
LA  - eng
PT  - Journal Article
DEP - 20160930
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Caproates)
RN  - 0 (Ghrelin)
RN  - 0 (Lactones)
RN  - 56RE988L1R (caprolactone)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - *Caproates
MH  - Disease Models, Animal
MH  - Ghrelin/*pharmacology
MH  - Guided Tissue Regeneration/*methods
MH  - *Lactones
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - *Nerve Regeneration/drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Recovery of Function/drug effects/physiology
EDAT- 2016/10/01 06:00
MHDA- 2017/08/16 06:00
CRDT- 2016/10/01 06:00
PHST- 2016/10/01 06:00 [pubmed]
PHST- 2017/08/16 06:00 [medline]
PHST- 2016/10/01 06:00 [entrez]
AID - HH-11-828 [pii]
AID - 10.14670/HH-11-828 [doi]
PST - ppublish
SO  - Histol Histopathol. 2017 Jun;32(6):627-637. doi: 10.14670/HH-11-828. Epub 2016 
      Sep 30.