PMID- 27687652
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20211204
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 36
IP  - 9
DP  - 2016 Aug 20
TI  - [P38 MAPK signaling pathway mediates advanced oxidation protein product-induced 
      epithelial-to-mesenchymal transition in tubular cells].
PG  - 1209-1214
AB  - OBJECTIVE: To investigate whether the p38 mitogen-activated protein kinase (MAPK) 
      signaling pathway mediates advanced oxidation protein products (AOPPs)-induced 
      epithelial-to-mesenchymal transition (EMT) in tubular cells. METHODS: Human 
      proximal tubular cells (HK-2 cells) exposed to AOPP-bovine serum albumin (BSA) 
      were examined for expressions of p38 MAPK and phosphorylated p38 MAPK using 
      Western blotting. Western blotting and quantitative RT-PCR were used to examine 
      the protein and mRNA expressions of EMT markers E-cadherin and vimentin and 
      endoplasmic reticulum stress marker glucose-regulated protein (GRP) 78 in cells 
      treated with SB203580 (an inhibitor of the p38 MAPK signaling pathway) prior to 
      AOPP exposure. The cells treated with AOPPs following pretreatment with 
      salubrinal (an inhibitor of endoplasmic reticulum stress) were also examined for 
      expressions of p38 MAPK and phosphorylated p38 MAPK. RESULTS: AOPP treatment 
      induced the phosphorylation of p38 MAPK in HK-2 cells. AOPP-induced decrease in 
      E-cadherin expression and overexpression of vimentin and GRP78 were partly 
      inhibited by pretreatment of the cells with SB203580. Salubrina partly suppressed 
      AOPP-induced phosphorylation of p38 MAPK in the cells. CONCLUSION: p38 MAPK 
      signaling pathway, which is regulated by endoplasmic reticulum stress, might 
      mediate AOPP-induced EMT in HK-2 cells.
FAU - Huang, Li-Li
AU  - Huang LL
AD  - Department of Nephrology, Fifth Affiliated Hospital, Southern Medical University, 
      Guangzhou 510900, China.E-mail: 396405501@qq.com.
FAU - Zhu, Xiao-Lin
AU  - Zhu XL
FAU - Deng, Wei-Qian
AU  - Deng WQ
FAU - Duan, Na
AU  - Duan N
FAU - Liang, Xiu-Jie
AU  - Liang XJ
FAU - Wang, Yue
AU  - Wang Y
FAU - Guo, Ting-Ting
AU  - Guo TT
FAU - Shu, Shuang-Shuang
AU  - Shu SS
FAU - Xiang, Xiao-Hong
AU  - Xiang XH
FAU - Jiang, Ting-Ting
AU  - Jiang TT
FAU - Tang, Xun
AU  - Tang X
FAU - Zhang, Jun
AU  - Zhang J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Advanced Oxidation Protein Products)
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cinnamates)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (Vimentin)
RN  - 0 (salubrinal)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - GYV9AM2QAG (Thiourea)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Advanced Oxidation Protein Products/*metabolism
MH  - Antigens, CD
MH  - Cadherins/metabolism
MH  - Cell Line
MH  - Cinnamates/pharmacology
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress
MH  - *Epithelial-Mesenchymal Transition
MH  - Heat-Shock Proteins/metabolism
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - *MAP Kinase Signaling System
MH  - Phosphorylation
MH  - Pyridines/pharmacology
MH  - Thiourea/analogs & derivatives/pharmacology
MH  - Vimentin/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2016/10/01 06:00
MHDA- 2018/10/16 06:00
CRDT- 2016/10/01 06:00
PHST- 2016/10/01 06:00 [entrez]
PHST- 2016/10/01 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2016 Aug 20;36(9):1209-1214.