PMID- 27688105
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20220409
IS  - 1879-0593 (Electronic)
IS  - 1368-8375 (Linking)
VI  - 61
DP  - 2016 Oct
TI  - Low etiologic fraction for human papillomavirus in larynx squamous cell 
      carcinoma.
PG  - 55-61
LID - S1368-8375(16)30137-3 [pii]
LID - 10.1016/j.oraloncology.2016.08.009 [doi]
AB  - BACKGROUND: Human Papillomavirus (HPV) is a cause of oropharyngeal squamous cell 
      carcinoma (OPSCC), but its pathogenic role in larynx squamous cell carcinoma 
      (LSCC) remains unclear. MATERIAL AND METHODS: A single-institutional, 
      retrospective case-series was performed to estimate the etiological fraction (EF) 
      for HPV in LSCC. Eligible cases included 436 consecutive cases of LSCC diagnosed 
      (2005-2014) at The Ohio State University Medical Center. HPV DNA presence was 
      detected by consensus primer PCR (Inno-LiPa) and HPV type-specific qPCR. HPV 
      E6/E7 mRNA expression was detected by type-specific qRT-PCR. Tumor p16 expression 
      was evaluated by immunohistochemistry (IHC). RESULTS: HPV DNA was detected by 
      Inno-LiPa in 54 of 404 (13.4%, 95% CI 10.2-17.1) evaluable samples but was 
      confirmed by HPV type-specific qPCR in only 14 (3.5%, 95% CI 1.9-5.7). Only 7 of 
      404 (1.7%, 95% CI 0.7-3.5) LSCC were positive for HPV E6/E7 mRNA expression, 
      including HPV16 (n=4) and 1 each for 11, 26 and 33. In the HPV11-positive tumor, 
      Sanger sequencing discovered 6 nucleotide mutations in the upstream regulation 
      region, E6 and E7. Of 404 LSCC, 18 had strong and diffuse p16 expression. In 
      comparison to a gold standard of HPV E6/E7 mRNA expression, p16 expression had a 
      sensitivity of 71.4% (95% CI 29.0-96.3), specificity of 96.7% (95% CI 94.5-98.3), 
      positive-predictive-value (PPV) of 27.8% (95% CI 9.7-53.5) and 
      negative-predictive-value of 99.5% (95% CI 98.1-99.9). CONCLUSION: The EF for HPV 
      in LSCC is low (1.7%) in a geographic region with high EF for OPSCC. Low-risk HPV 
      may rarely cause LSCC. Finally, p16 expression has poor PPV for HPV in LSCC.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Taberna, M
AU  - Taberna M
AD  - Department of Medical Oncology, Catalan Institute of Oncology (ICO), IDIBELL, 
      L'Hospitalet de Llobregat, Barcelona, Spain; Cancer Epidemiology Research 
      Program, Catalan Institute of Oncology (ICO), IDIBELL, L'Hospitalet de Llobregat, 
      Barcelona, Spain; University of Barcelona, Barcelona, Spain.
FAU - Resteghini, C
AU  - Resteghini C
AD  - Head and Neck Medical Oncology Department, Fondazione IRCCS Istituto Nazionale 
      Tumori, Milan, Italy.
FAU - Swanson, B
AU  - Swanson B
AD  - Department of Pathology, The Ohio State University Medical Center, Columbus, OH, 
      United States.
FAU - Pickard, R K L
AU  - Pickard RK
AD  - Department of Medicine, The Ohio State University, Columbus, OH, United States.
FAU - Jiang, B
AU  - Jiang B
AD  - Department of Medicine, The Ohio State University, Columbus, OH, United States.
FAU - Xiao, W
AU  - Xiao W
AD  - Department of Medicine, The Ohio State University, Columbus, OH, United States.
FAU - Mena, M
AU  - Mena M
AD  - Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), 
      IDIBELL, L'Hospitalet de Llobregat, Barcelona, Spain.
FAU - Kreinbrink, P
AU  - Kreinbrink P
AD  - Department of Medicine, The Ohio State University, Columbus, OH, United States.
FAU - Chio, E
AU  - Chio E
AD  - Department of Otolaryngology, The Ohio State University Medical Center, Columbus, 
      OH, United States.
FAU - Gillison, M L
AU  - Gillison ML
AD  - Department of Medicine, The Ohio State University, Columbus, OH, United States. 
      Electronic address: maura.gillison@osumc.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160831
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
SB  - IM
MH  - Alphapapillomavirus/*isolation & purification
MH  - Carcinoma, Squamous Cell/*virology
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*virology
MH  - Male
OTO - NOTNLM
OT  - HPV DNA PCR
OT  - HPV E6/E7 mRNA qRT-PCR
OT  - HPV11
OT  - Head and neck cancer
OT  - Head and neck squamous cell carcinoma
OT  - Human papillomavirus
OT  - Larynx cancer
OT  - p16 immunohistochemistry
EDAT- 2016/10/01 06:00
MHDA- 2018/02/07 06:00
CRDT- 2016/10/01 06:00
PHST- 2016/06/27 00:00 [received]
PHST- 2016/08/08 00:00 [revised]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/10/01 06:00 [entrez]
PHST- 2016/10/01 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
AID - S1368-8375(16)30137-3 [pii]
AID - 10.1016/j.oraloncology.2016.08.009 [doi]
PST - ppublish
SO  - Oral Oncol. 2016 Oct;61:55-61. doi: 10.1016/j.oraloncology.2016.08.009. Epub 2016 
      Aug 31.