PMID- 27690722
OWN - NLM
STAT- MEDLINE
DCOM- 20180322
LR  - 20220409
IS  - 2000-1967 (Electronic)
IS  - 0300-9734 (Print)
IS  - 0300-9734 (Linking)
VI  - 122
IP  - 1
DP  - 2017 Mar
TI  - Differential neutrophil chemotactic response towards IL-8 and bacterial N-formyl 
      peptides in term newborn infants.
PG  - 35-42
LID - 10.1080/03009734.2016.1228721 [doi]
AB  - BACKGROUND: A prerequisite for an effective innate immunity is the migrative 
      ability of neutrophils to respond to inflammatory and infectious agents such as 
      the intermediate interleukin (IL)-8 and the end-target 
      formyl-methionyl-leucyl-phenylalanine (fMLP) chemoattractants. The aim was to 
      study the chemotactic capacity of neutrophils from newborn infants and adults in 
      response to IL-8 and the bacterial peptide fMLP. METHODS: In the under-agarose 
      cell migration assay, isolated leukocytes from healthy adults and from cord blood 
      of healthy term newborn infants were studied with dose responses towards IL-8 and 
      fMLP. The same number of leukocytes (1 x 10(5) cells), with the same distribution 
      of neutrophils and monocytes, were analyzed in neonates and adults. Chemotaxis 
      was distinguished from randomly migrating neutrophils, and the neutrophil pattern 
      of migration, i.e. the migration distance and the number of migrating neutrophils 
      per distance, was evaluated. RESULTS: In comparison to adults, fewer neutrophils 
      from newborn infants migrated towards IL-8 and for a shorter distance (P < .01, 
      respectively). The number of neutrophils migrating to different gradients of 
      fMLP, the distance they migrated, and the correlation between the number and the 
      distance were the same for neonates and adults. Random migration did not differ 
      in any instance. CONCLUSION: Chemotaxis of neutrophils from newborn infants was 
      as co-ordinated as neutrophils from adults in response to fMLP, whereas the 
      response to IL-8 was reduced. The differential response of neutrophils from 
      neonates to intermediate and end-target chemoattractants could indicate a reduced 
      infectious response.
FAU - Stalhammar, Maria E
AU  - Stalhammar ME
AD  - a Department of Women's and Children's Health , Uppsala University , Uppsala , 
      Sweden.
FAU - Douhan Hakansson, Lena
AU  - Douhan Hakansson L
AD  - b Department of Medical Sciences , Uppsala University , Uppsala , Sweden.
FAU - Jonzon, Anders
AU  - Jonzon A
AD  - a Department of Women's and Children's Health , Uppsala University , Uppsala , 
      Sweden.
FAU - Sindelar, Richard
AU  - Sindelar R
AD  - a Department of Women's and Children's Health , Uppsala University , Uppsala , 
      Sweden.
LA  - eng
PT  - Journal Article
DEP - 20161003
PL  - Sweden
TA  - Ups J Med Sci
JT  - Upsala journal of medical sciences
JID - 0332203
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-8)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cell Movement
MH  - Chemotactic Factors/chemistry
MH  - *Chemotaxis
MH  - Humans
MH  - Immunity, Innate
MH  - Infant, Newborn
MH  - Interleukin-8/*chemistry
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/*chemistry
MH  - Neutrophils/*cytology
MH  - Sepharose/chemistry
MH  - Young Adult
PMC - PMC5361430
OTO - NOTNLM
OT  - Chemotaxis
OT  - IL-8
OT  - chemoattractants
OT  - fMLP
OT  - innate immunity
OT  - neutrophils
OT  - newborn infants
EDAT- 2016/10/04 06:00
MHDA- 2018/03/23 06:00
PMCR- 2017/03/01
CRDT- 2016/10/04 06:00
PHST- 2016/10/04 06:00 [pubmed]
PHST- 2018/03/23 06:00 [medline]
PHST- 2016/10/04 06:00 [entrez]
PHST- 2017/03/01 00:00 [pmc-release]
AID - iups-122-35 [pii]
AID - 10.1080/03009734.2016.1228721 [doi]
PST - ppublish
SO  - Ups J Med Sci. 2017 Mar;122(1):35-42. doi: 10.1080/03009734.2016.1228721. Epub 
      2016 Oct 3.