PMID- 27692393
OWN - NLM
STAT- MEDLINE
DCOM- 20181026
LR  - 20181026
IS  - 2255-5021 (Electronic)
IS  - 2255-5021 (Linking)
VI  - 56
IP  - 5
DP  - 2016 Sep-Oct
TI  - Clinical characteristics and frequency of TLR4 polymorphisms in Brazilian 
      patients with ankylosing spondylitis.
PG  - 432-440
LID - S2255-5021(16)30046-3 [pii]
LID - 10.1016/j.rbre.2016.07.004 [doi]
AB  - OBJECTIVES: Innate immunity is involved in the physiopathology of ankylosing 
      spondylitis (AS), with the participation of Gram-negative bacteria, modulation of 
      human leukocyte antigen (HLA) B27 and the involvement of pattern recognition 
      receptors, such as Toll-like receptors (TLRs). The aim of this study was to 
      investigate the clinical characteristics and frequency of TLR4 polymorphisms 
      (Asp299Gly and Thr 399Ile) in a cohort of Brazilian patients with AS. METHODS: A 
      cross-sectional study was carried out involving 200 patients with a diagnosis of 
      AS and a healthy control group of 200 individuals. Disease activity, severity and 
      functional capacity were measured. The study of TLR4 polymorphisms was performed 
      using the restriction fragment length polymorphism method. HLA-B27 was analyzed 
      by conventional polymerase chain reaction. The IBM SPSS Statistics 20 program was 
      used for the statistical analysis, with p-values less than 0.05 considered 
      significant. RESULTS: Mean age and disease duration were 43.1+/-12.7 and 16.6+/-9.2 
      years, respectively. The sample was predominantly male (71%) and non-Caucasian 
      (52%). A total of 66% of the group of patients were positive for HLA-B27. The 
      sample of patients was characterized by moderate functional impairment and a high 
      degree of disease activity. No significant association was found between the two 
      TLR4 polymorphisms and susceptibility to AS. CONCLUSIONS: TLR4 polymorphisms 399 
      and 299 were not more frequent in patients with AS in comparison to the health 
      controls and none of the clinical variables were associated with these 
      polymorphisms.
CI  - Copyright (c) 2016 Elsevier Editora Ltda. All rights reserved.
FAU - Machado, Natalia Pereira
AU  - Machado NP
AD  - Divisao de Reumatologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Nogueira, Eliana
AU  - Nogueira E
AD  - Divisao de Nefrologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Oseki, Karen
AU  - Oseki K
AD  - Divisao de Reumatologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Ebbing, Pamela Carolina Cruz
AU  - Ebbing PC
AD  - Divisao de Reumatologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Origassa, Clarice Silvia Taemi
AU  - Origassa CS
AD  - Divisao de Nefrologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Mohovic, Tatiane
AU  - Mohovic T
AD  - Divisao de Nefrologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Camara, Niels Olsen Saraiva
AU  - Camara NO
AD  - Divisao de Nefrologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Pinheiro, Marcelo de Medeiros
AU  - Pinheiro MM
AD  - Divisao de Reumatologia, Departamento de Medicina, Universidade Federal de Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil. Electronic address: mpinheiro@uol.com.br.
LA  - eng
LA  - por
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160805
PL  - Brazil
TA  - Rev Bras Reumatol Engl Ed
JT  - Revista brasileira de reumatologia
JID - 101672232
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Brazil
MH  - Case-Control Studies
MH  - Cross-Sectional Studies
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - HLA-B27 Antigen/*genetics
MH  - Humans
MH  - Male
MH  - Polymorphism, Restriction Fragment Length/*genetics
MH  - Spondylitis, Ankylosing/*genetics
MH  - Toll-Like Receptor 4/*genetics
OTO - NOTNLM
OT  - Ankylosing spondylitis
OT  - Espondilite anquilosante
OT  - HLA-B27
OT  - Polimorfismos em TLR-4
OT  - TLR-4 polymorphisms
EDAT- 2016/10/04 06:00
MHDA- 2018/10/27 06:00
CRDT- 2016/10/04 06:00
PHST- 2016/02/10 00:00 [received]
PHST- 2016/05/01 00:00 [accepted]
PHST- 2016/10/04 06:00 [entrez]
PHST- 2016/10/04 06:00 [pubmed]
PHST- 2018/10/27 06:00 [medline]
AID - S2255-5021(16)30046-3 [pii]
AID - 10.1016/j.rbre.2016.07.004 [doi]
PST - ppublish
SO  - Rev Bras Reumatol Engl Ed. 2016 Sep-Oct;56(5):432-440. doi: 
      10.1016/j.rbre.2016.07.004. Epub 2016 Aug 5.